Retinoic acid inhibits tumor and normal liver cells proliferation in culture (2004)
- Authors:
- Autor USP: SANTELLI, GLAUCIA MARIA MACHADO - ICB
- Unidade: ICB
- Assunto: HISTOLOGIA
- Language: Inglês
- Abstract: In the last decades alternative therapies have been search for the treatment of the liver cancer to improve the probability of cure for the disease. Several studies investigated the biological activity of the retinoids in different tumor cell lines, since they act on the regulation of the cell proliferation and differentiation processes. Retinoic acid, a biologically active metabolite of vitamin A play a critical role during normal development and regulates growth and/or differentiation in a variety of tumor cell lines. The present work investigated the effect of the retinoic acid on the proliferation in normal and tumoral liver cell lines. The results obtained by the "kit CellTiter 96R AQueousNon- Radioative Cell Proliferation Assay" (MTS) after the treatment with retinoic acid (10-7 M) for 48h plus 24h of recovery in normal medium showed 38% proliferation inhibition in the normal cells and 45% in the tumoral ones. Flow cytometry (FACS) analysis indicated that G0/G1 cell population decreases drastically with 48h of recovery as well as polyploid cell population increased. The immunofluoresce assay showed morphologic alterations in normal cells after the treatment. The cell organization in culture resembled that of hepatic lobules presenting an increased number of binucleated cells. Present data agree with the suggestion of the retinoic acid used as an alternative therapy in the treatment of the cancer, including hepatic tumors
- Imprenta:
- Source:
- Título: Abstracts
- Conference titles: Congress of the Brazilian Society for Cell Biology
-
ABNT
IONTA, Marisa e MACHADO-SANTELLI, Gláucia Maria. Retinoic acid inhibits tumor and normal liver cells proliferation in culture. 2004, Anais.. Campinas: Instituto de Ciências Biomédicas, Universidade de São Paulo, 2004. . Acesso em: 06 nov. 2024. -
APA
Ionta, M., & Machado-Santelli, G. M. (2004). Retinoic acid inhibits tumor and normal liver cells proliferation in culture. In Abstracts. Campinas: Instituto de Ciências Biomédicas, Universidade de São Paulo. -
NLM
Ionta M, Machado-Santelli GM. Retinoic acid inhibits tumor and normal liver cells proliferation in culture. Abstracts. 2004 ;[citado 2024 nov. 06 ] -
Vancouver
Ionta M, Machado-Santelli GM. Retinoic acid inhibits tumor and normal liver cells proliferation in culture. Abstracts. 2004 ;[citado 2024 nov. 06 ] - Cytoskeleton and nuclear DNA content in pleomorphic cell cultures
- Labeling pattern of M30 antibody during apoptotic process induced by UV radiation
- Reconstrução 3D: interação entre células de mamíferos e crisotila
- Salivary system in leaf-cutting ants (Atta sexdens rubropilosa): a confocal analyses
- Estudo comparativo do dna de tumores de mama humana e de suas respectivas metastases ganglionares
- Interdependencia dos processos de proliferacao e comunicacao intercelular via juncoes comunicantes em celulas epiteliais de figado de rato, in vitro
- Frequencia de micronucleos em celulas sem atipias, exfoliadas do colo uterino
- Cytoskeletal alterations in cells treated with melatonin
- Gap junction intercellular communication, DNA ploidy and proliferation in hepatocytes in culture
- Effects of vincristin on cultured cells and its association with aneuploidy induction
How to cite
A citação é gerada automaticamente e pode não estar totalmente de acordo com as normas
