Labeling pattern of M30 antibody during apoptotic process induced by UV radiation (2004)
- Authors:
- Autor USP: SANTELLI, GLAUCIA MARIA MACHADO - ICB
- Unidade: ICB
- Assunto: HISTOLOGIA
- Language: Inglês
- Abstract: The present study focused the dynamic of apoptosis process in monolayer cell culture. The monoclonal antibody M30, specific for the neo-epitope in cytokeratin 18 that becomes available at an early caspase cleavage event during apoptosis, can be utilized specifically to recognize apoptotic epithelial cells. Indeed, cytoskeleton proteolytic cleavage occurs before DNA strand breaking and plasma membrane blebbing. The ultraviolet (UV) radiation is an environmental agent that leads to apoptosis in epidermal cells, probably as a process to rid potentially cancerous cells originated by mutagenic UV irradiation. The BBnt (normal epithelial bovine cell bladder) cells showed a high level of apoptosis induction (77,6% of cells) 24 hours after UV radiation for 1 minute. Typical nuclear apoptotic morphology was observed in those cells when they were stained with propidium iodide or DAPI. Immunofluorescence using M30 antibody was performed to detect early apoptosis phases in the monolayer cells compared with those in suspension. M30 labeling pattern analyzed by confocal microscopy was associated with the kinetics of apoptosis induction
- Imprenta:
- Source:
- Título do periódico: Abstracts
- Conference titles: Congress of the Brazilian Society for Cell Biology
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ABNT
NAKAGAWA, Elly Kayoko; URABAYASHI, Marcel Shiniti; CORTEZ, Beatriz de Araujo; MACHADO-SANTELLI, Gláucia Maria. Labeling pattern of M30 antibody during apoptotic process induced by UV radiation. Anais.. Campinas: [s.n.], 2004. -
APA
Nakagawa, E. K., Urabayashi, M. S., Cortez, B. de A., & Machado-Santelli, G. M. (2004). Labeling pattern of M30 antibody during apoptotic process induced by UV radiation. In Abstracts. Campinas. -
NLM
Nakagawa EK, Urabayashi MS, Cortez B de A, Machado-Santelli GM. Labeling pattern of M30 antibody during apoptotic process induced by UV radiation. Abstracts. 2004 ; -
Vancouver
Nakagawa EK, Urabayashi MS, Cortez B de A, Machado-Santelli GM. Labeling pattern of M30 antibody during apoptotic process induced by UV radiation. Abstracts. 2004 ; - Effects of vincristin on cultured cells and its association with aneuploidy induction
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