Stimulation of early response genes in rat adrenal primary culture cells treated with acth and FGF2 (2004)
- Authors:
- Autor USP: LOTFI, CLAUDIMARA FERINI PACICCO - ICB
- Unidade: ICB
- Assunto: ANATOMIA
- Language: Inglês
- Abstract: Introduction and Goals: Mammalian cell cycle control is exerted at the G0-G1-S transition by hormones and growth factors inducing early response genes, fos and jun protooncogene families. FGF2 (fibroblast growth factor) mimic the mitogenic actions of growth factors in Y1 cell line while ACTH (adrenocorticotropin) does not present signaling potential sufficient to this response. We analyzed the kinetic of regulation of expression of Fos and Jun proteins in rat primary culture cells, treated by ACTH or/and FGF2. Methods: Adrenal primary culture cells, glomerulosa (G) and fasciculata/reticularis (F/R) cells were obtained from Wistar male rats through and seeded in DMEM medium+10%FCS at 37ºC and 5%C02. After serum-starved the cells were treated with 10-9M ACTH(A), 1ng/ml FGF2(F) or A+F for different times and analyzed by immunoperoxidase or Western Blotting. Results and Conclusion: cFos proteins are not detected in the nuclei serum starved cells but is induced by ACTH and FGF in G and F/R cells with the same kinetic, with peaking at 2h, but with different intensity. FGF2 induction reaches 50% of the level attained upon ACTH induction. A+F presented maximal cFos activation in labeled nuclei but present an additive effect on protein expression. The FGF2 is poor inductor of cFos protein however A+F combination results suggest that FGF2 may have an important role in the growth response of ACTH in adrenal cells
- Imprenta:
- Source:
- Título: Abstracts
- Conference titles: Congress of the Brazilian Society for Cell Biology
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ABNT
VIEIRA, L. R. e LOTFI, Claudimara Ferini Pacicco. Stimulation of early response genes in rat adrenal primary culture cells treated with acth and FGF2. 2004, Anais.. Campinas: Instituto de Ciências Biomédicas, Universidade de São Paulo, 2004. . Acesso em: 07 out. 2024. -
APA
Vieira, L. R., & Lotfi, C. F. P. (2004). Stimulation of early response genes in rat adrenal primary culture cells treated with acth and FGF2. In Abstracts. Campinas: Instituto de Ciências Biomédicas, Universidade de São Paulo. -
NLM
Vieira LR, Lotfi CFP. Stimulation of early response genes in rat adrenal primary culture cells treated with acth and FGF2. Abstracts. 2004 ;[citado 2024 out. 07 ] -
Vancouver
Vieira LR, Lotfi CFP. Stimulation of early response genes in rat adrenal primary culture cells treated with acth and FGF2. Abstracts. 2004 ;[citado 2024 out. 07 ] - Involvement of map kinases pathway mediated by acth and FGF2 in rat adrenal primary culture cells
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