Expression of a treptococcal M protein epitope fused at different sites of flagellin (FliCd) as an approach for the development of bivalent vaccines based on attenuated Salmonella strains (2003)
- Authors:
- Autor USP: FERREIRA, LUIS CARLOS DE SOUZA - ICB
- Unidade: ICB
- Assunto: MICROBIOLOGIA
- Language: Inglês
- Abstract: Flagellin, the flagellar filament monomer, has been used as a carrier for heterologous epitopes genetically inserted at the central hypervariable region (region IV). Recombinant flagellins are often functional and can elicit humoral responses to both flagellin and heterologous epitope in mice following parenteral immunization. In the present work we investigated the immunogenicity and vaccine potential of attenuated Salmonella strains able to express recombinant flagellins genetically fused at different sites to a 15 mer linear epitope of Streptococcus pyogenes M5 protein as an approach to identify alternative sites for the integration of heterologous epitopes at the FliCd flagellin. The peptide was inserted at flagellin regions presenting reduced (region IV), partial (region III and VI) or complete homology (region VIII) among several fliC alleles. All regions, with exception of the highly conserved region VIII, accepted the insertions without loss of motility, albeit, in some cases, motility was significantly reduced during in vitro growth. Only mice immunized parenterally with live Salmonella strains expressing heterologous flagellin fused with the M5 protein epitope at region IV and VIII were fully protected after challenge with S. pyogenes. The same recombinant flagellins with fusions at regions IV and VIII were also able to elicit serum antibody responses to the heterologous peptide as determined in ELISA assays. In contrast to fusions at other flagellinintegration sites, Salmonella strains expressing recombinant flagellins with the insert at region VIII produced soluble molecules unable to assemble into morphological normal flagella. Based on the present evidences we suggest that regions other regions besides region IV can also be used for the genetic fusion of heterologous epitopes at the Salmonella flagellin as an approach to engender peptide-based bivalent vaccines. These results further demonstrated that expression of epitopes derived from the M5 protein genetically fused to Salmonella flagellin represents a promising alternative for the development of anti-streptococcus vaccines without the risk to elicit autoimmune responses
- Imprenta:
- Publisher: Sociedade Brasileira de Microbiologia
- Date published: 2003
- Conference titles: Congresso Brasileiro de Microbiologia
-
ABNT
AZEVEDO, F. P. M. et al. Expression of a treptococcal M protein epitope fused at different sites of flagellin (FliCd) as an approach for the development of bivalent vaccines based on attenuated Salmonella strains. 2003, Anais.. [S.l.]: Sociedade Brasileira de Microbiologia, 2003. . Acesso em: 27 dez. 2025. -
APA
Azevedo, F. P. M., Sbrogio-Almeida, M. E., Newton, S. M. C., & Ferreira, L. C. de S. (2003). Expression of a treptococcal M protein epitope fused at different sites of flagellin (FliCd) as an approach for the development of bivalent vaccines based on attenuated Salmonella strains. In . Sociedade Brasileira de Microbiologia. -
NLM
Azevedo FPM, Sbrogio-Almeida ME, Newton SMC, Ferreira LC de S. Expression of a treptococcal M protein epitope fused at different sites of flagellin (FliCd) as an approach for the development of bivalent vaccines based on attenuated Salmonella strains. 2003 ;[citado 2025 dez. 27 ] -
Vancouver
Azevedo FPM, Sbrogio-Almeida ME, Newton SMC, Ferreira LC de S. Expression of a treptococcal M protein epitope fused at different sites of flagellin (FliCd) as an approach for the development of bivalent vaccines based on attenuated Salmonella strains. 2003 ;[citado 2025 dez. 27 ] - Bivalent DNA vaccines confer prophylactic and therapeutic effect on HPV-16 murine tumor
- Evaluation of the prophylactic and therapeutic effects of anti-cancer DNA vaccine encoding the HPV-16 E7 oncoprotein genetically fused to the HSV-1 GD
- Protective immune response in mice immunized with the GP43-derived P10 peptide and Salmonella enterica FLICD flagellin
- Immunomodulatory properties of the enterotoxigenic Escherichia coli type IIa heat-labile enterotoxin (LT-IIa) in mice immunized via the intradermal route
- Protective Immune Responses to enteropathogenic Escherichia coli Strain with a New Recombinant Bacillus subtilis Vaccine strains
- Modulação da resposta imune pela listeriolisina o (LLO) expressa por linhagens recombinantes de bacillus subtilis empregadas como vetores vacinais vivos
- Evidence of ball-and-chain transport of ferric enterobactin through FepA
- Effect of gender on training-induced vascular remodeling in SHR
- Enhanced anti-tumor effect of a gene gun-delivered DNA vaccine encoding the human papillomavirus type 16 oncoproteins genetically fused to the herpes simplex virus glycoprotein D
- Dengue virus models based on mice as experimental hosts
How to cite
A citação é gerada automaticamente e pode não estar totalmente de acordo com as normas
