Bivalent DNA vaccines confer prophylactic and therapeutic effect on HPV-16 murine tumor (2003)
- Authors:
- USP affiliated author: FERREIRA, LUIS CARLOS DE SOUZA - ICB
- School: ICB
- Subject: MICROBIOLOGIA
- Language: Inglês
- Abstract: An important goal of immunotherapy against human papillomavirus (HPV) infection and cervical cancer is control of tumor growth. Here, we constructed bivalent DNA vaccines based on genetic fusion of HPV-16 E6 and E7 oncoproteins and glycoprotein D of HSV-1 (gD). Plasmids pRE4E7 and pRE4E6E7, encoding E7/gD and E6/E7/gD, respectively, were able to promote in vitro transcription of specific mRNAs and express the hybrid proteins. The DNA vaccines induced specific IFN-g CD8-dependent T cell responses in vaccinated female C57/Bl6 mice. Animals immunized with four doses of either pRE4E7 or pRE4E6E7 were complete protected to challenges with malignant TC-1 cells in a murine HPV-16-induced cancer model. We additionally evaluated levels of immunotherapeutic effect the bivalent DNA vaccines as determined in mice vaccinated on the same day or 5 days following the implantation of the malignant TC1 cells. The results indicated that both vaccine formulations were able to eradicate tumors from 40% the immunized animals. In conclusion, we showed that bivalent DNA vaccines expressing HPV-16 oncoproteins are promising candidates for therapeutic and prophylactic interventions of tumors induced by HPV-16
- Imprenta:
- Publisher: Sociedade Brasileira de Microbiologia
- Date published: 2003
- Conference title: Congresso Brasileiro de Microbiologia
-
ABNT
LASARO, M. O.; FERREIRA, Luís Carlos de Souza; DINIZ, M. O.; ERTL, H. C. J. Bivalent DNA vaccines confer prophylactic and therapeutic effect on HPV-16 murine tumor. Anais.. [S.l.]: Sociedade Brasileira de Microbiologia, 2003. -
APA
Lasaro, M. O., Ferreira, L. C. de S., Diniz, M. O., & Ertl, H. C. J. (2003). Bivalent DNA vaccines confer prophylactic and therapeutic effect on HPV-16 murine tumor. In . Sociedade Brasileira de Microbiologia. -
NLM
Lasaro MO, Ferreira LC de S, Diniz MO, Ertl HCJ. Bivalent DNA vaccines confer prophylactic and therapeutic effect on HPV-16 murine tumor. 2003 ; -
Vancouver
Lasaro MO, Ferreira LC de S, Diniz MO, Ertl HCJ. Bivalent DNA vaccines confer prophylactic and therapeutic effect on HPV-16 murine tumor. 2003 ; - Construção de uma vacina de DNA bivalente contra os vírus do papiloma e herpes simplex humano
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