Preliminary assessment of bacterial community by PCR-DGGE in antartic soils (2003)
- Authors:
- Autor USP: PELLIZARI, VIVIAN HELENA - ICB
- Unidade: ICB
- Assunto: MICROBIOLOGIA
- Language: Inglês
- Abstract: Antarctica is considered as one of the last pristine areas on Earth and is protected by the Antarctic Treaty (Washington, 1959). However, even precaution and security procedures are adopted regularly to prevent accidents, oil spillage still can occur during field operations. In the present study, 12 Antarctic soil samples were collected from areas surrounding the diesel fuel storage tanks near the Brazilian Antarctic Station (EACF) and from other non-contaminated sites distributed along Admiralty and Maxwell Bays, King George Island, Antarctic Peninsula. The bacterial community was analyzed by enumeration of total heterotrophic and potential oil-degraders at 4°C and 20°C, and by PCR-DGGE analysis based on 16S ribosomal DNA sequence. Total community DNA was isolated and purified with PVPP. The DNA extracts were used to amplify a conserved region of the 16S rDNA by PCR. Twenty-eight stronger bands produced by DGGE were extracted from the gels and sequenced directly. DGGE fragments which showed more than one band after re-amplification were cloned and then sequenced. Results of total heterotrophics and potential oil-degraders found in Antarctic soils, were two orders of magnitude higher around diesel tanks than in other areas, probably because the previous exposition of microorganisms on oil contamination. No differences about the growth temperatures 4ºC and 20ºC were observed among the bacterial population, indicating that the Antarctic cold-adapted bacterial communitiesare more psychrotrophic rather than psychrophilic, similar to those populations found in Arctic and Alpine soils. The DGGE showed distinct profiles to the bacterial communities from contaminated and non-contaminated soils, with a higher number of strongest bands proceeding from the contaminated sites. Sequencing analysis revealed organisms such as P. solanacearum, Burkholderia sp. and M. aurum. Our studies showed that oil-contamination probably lead to changes in the community structure and in bacterial diversity
- Imprenta:
- Publisher: Sociedade Brasileira de Microbiologia
- Date published: 2003
- Conference titles: Congresso Brasileiro de Microbiologia
-
ABNT
LUZ, A. P. et al. Preliminary assessment of bacterial community by PCR-DGGE in antartic soils. 2003, Anais.. [S.l.]: Sociedade Brasileira de Microbiologia, 2003. . Acesso em: 31 dez. 2025. -
APA
Luz, A. P., Fortin, N., Greer, C. W., & Pellizari, V. H. (2003). Preliminary assessment of bacterial community by PCR-DGGE in antartic soils. In . Sociedade Brasileira de Microbiologia. -
NLM
Luz AP, Fortin N, Greer CW, Pellizari VH. Preliminary assessment of bacterial community by PCR-DGGE in antartic soils. 2003 ;[citado 2025 dez. 31 ] -
Vancouver
Luz AP, Fortin N, Greer CW, Pellizari VH. Preliminary assessment of bacterial community by PCR-DGGE in antartic soils. 2003 ;[citado 2025 dez. 31 ] - Aerobiology over Antarctica: a new initiative for atmospheric ecology
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