Detection of norovirus and astrovirus mixed infections in stool samples of children with gastroenteritis from São Paulo, Brazil (2003)
- Authors:
- Autor USP: RACZ, MARIA LUCIA BARBOSA DE OLIVEIRA - ICB
- Unidade: ICB
- Assunto: MICROBIOLOGIA
- Language: Português
- Abstract: Objetivo:Norovirus and astrovirus are recognised as important etiologic agents of viral gastroenteritis. Noroviruses are a common cause of both outbreakes and sporadic cases of human viral gastroenteritis and astroviruses have been recognised as important agents in paediatric diarrhoea. The noroviruses are classified within the family Caliciviridae, viral particles are small (27-40nm), non-enveloped, with icosahedral symmetry. The genome is a positive sense single strand RNA (ssRNA), with approximately 7,4-7,7 kb in size. Belonging to the Astroviridae family, astrovirus is a small spherical (28-30 nm) non-enveloped virus, with icosahedral symmetry and a star-like capsid. The genome is a positive sense single strand RNA (ssRNA), with approximately 6,8-7,2 kb. The objective of this study was to detect norovirus and astrovirus in 193 stool samples from São Paulo city, Brazil, collected from 12-day-old to 14-year-old children with gastroenteritis during a 5-year-period (1995-1999).Samples were previosly tested for rotavirus and adenovirus and were found negative for these viruses. Métodos e Resultados: The detection of noroviruses were performed by reverse transcription-polymerase chain reaction (RT-PCR), using primers MON 431, 432, 433 and 434, that amplify a 213 bp fragment from RNA polymerase gene. For astrovirus detection RT-PCR was also used, with primers MON 269 and MON 270, resulting in a amplification of 449 bp fragment whenpositive. Results showed that 75 (38,9 %) samples were found to be positive for norovirus, 60 (31,0%) were positive for astrovirus and 25 (13,0%) were positive for both viruses. Conclusão: This is the first report of mixed noroviruses/astrovirus infections in sporadic cases of gastroenteritis in children in Brazil. Results also show the importance of these viruses as agents of gastroenteritis
- Imprenta:
- Publisher: Comissão de Pesquisa do ICB/USP
- Publisher place: São Paulo
- Date published: 2003
- Source:
- Título: Resumos
- Conference titles: Congresso Instituto Ciências Biomédicas, IV
-
ABNT
CASTILHO, Juliana Galera et al. Detection of norovirus and astrovirus mixed infections in stool samples of children with gastroenteritis from São Paulo, Brazil. 2003, Anais.. São Paulo: Comissão de Pesquisa do ICB/USP, 2003. . Acesso em: 10 jan. 2026. -
APA
Castilho, J. G., Racz, M. L. B. de O., Resque, H. R., Munford, V., & Fagundes-Neto, U. (2003). Detection of norovirus and astrovirus mixed infections in stool samples of children with gastroenteritis from São Paulo, Brazil. In Resumos. São Paulo: Comissão de Pesquisa do ICB/USP. -
NLM
Castilho JG, Racz MLB de O, Resque HR, Munford V, Fagundes-Neto U. Detection of norovirus and astrovirus mixed infections in stool samples of children with gastroenteritis from São Paulo, Brazil. Resumos. 2003 ;[citado 2026 jan. 10 ] -
Vancouver
Castilho JG, Racz MLB de O, Resque HR, Munford V, Fagundes-Neto U. Detection of norovirus and astrovirus mixed infections in stool samples of children with gastroenteritis from São Paulo, Brazil. Resumos. 2003 ;[citado 2026 jan. 10 ] - Use of ultracentrifugation technique to increase sensitivity of RT-PCR genotyping of swine ratavirus
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