Molecular characterization of rotavirus in fecal samples of children from Teresina, Piauí, Brazil (2001)
- Authors:
- Autor USP: RACZ, MARIA LUCIA BARBOSA DE OLIVEIRA - ICB
- Unidade: ICB
- Subjects: VIROLOGIA; CIÊNCIAS (CONGRESSOS;CONFERENCIAS); ROTAVIRUS; FEZES (AMOSTRA); CRIANÇAS
- Language: Inglês
- Abstract: Acute infantile diarrhea is responsible for a large number of pediatric hospitalizations worldwide and for high infant mortality in developing countries. Rotavirus is the most frequent pathogen associated with diarrhea in young children.Objectives: The purpose of this work was to characterization samples from Teresina by RT-PCR. Methods and Results: In the present study we tested 24 rotavirus positive samples collected in the Hospital Infantil Lucídio Portela, Teresina, Piauí, from children from 0 to 5 years of age with diarrhea. Rotavirus was identified in stools by an enzyme immunoassay (EIARA-FIOCRUZ). The samples were then submitted to molecular characterization, using reverse transcription (RT) followed by polymerase chain reaction (PCR) with specific primers for the most common human and animals rotavirus genotypes. PCR results showed a high diversity of G and P genotypes. Samples were classified into common human G genotypes G[1], G[2] and G[4], with the majority of samples (41,7%) belonging to genotype G[1]. G[3] was not found. Genotype G[5], commonly found in porcine samples, was detected in 12,5% of the samples. Mixed G genotypes G[1][5] and G[2][5] were also detectedThe most common P genotype was P[8] (25%). P[4] was detected in 8,3% of the samples. Mixed P genotype P[1][8] was detected in 16,7% of the samples and P[6, M37][8], P[6, Gott][8] and P[4][6, M37][8] were also found. P[6] genotypes were designated as P[6, M37] (humanspecific) and P[6, Gott] (porcine specific). G[5] strains are common human pathogens in Brazil, usually combined with P[8] genotype. In the present study we found G[5] and mixed G[1][5] and G[2][5] combined with P[1][8], P[8] and P[6, Gott][8]. The majority of G[1] was combined with P[8] as expected but combinations with P[4], P[1][8] and P[6, M37][8] were also detected. P[1] genotype is commonly found in bovine samples. Conclusions: Combinations like G[5]P[8], G[5]P[1][8] and G[1]P[1][8] suggests the possibility of exchange of VP7/ VP4 genes between animal and human rotavirus and interspecies transmition of these viruses
- Imprenta:
- Publisher: Comissão de Cultura e Extensão Universitária do ICB/USP
- Publisher place: São Paulo
- Date published: 2001
- Source:
- Título: Resumos
- Conference titles: Congresso do Instituto de Ciências Biomédicas
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ABNT
MACEDO, C. I. et al. Molecular characterization of rotavirus in fecal samples of children from Teresina, Piauí, Brazil. 2001, Anais.. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2001. . Acesso em: 11 jan. 2026. -
APA
Macedo, C. I., Rácz, M. L. B. de O., Christofoletti, A., & Munford, V. (2001). Molecular characterization of rotavirus in fecal samples of children from Teresina, Piauí, Brazil. In Resumos. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP. -
NLM
Macedo CI, Rácz MLB de O, Christofoletti A, Munford V. Molecular characterization of rotavirus in fecal samples of children from Teresina, Piauí, Brazil. Resumos. 2001 ;[citado 2026 jan. 11 ] -
Vancouver
Macedo CI, Rácz MLB de O, Christofoletti A, Munford V. Molecular characterization of rotavirus in fecal samples of children from Teresina, Piauí, Brazil. Resumos. 2001 ;[citado 2026 jan. 11 ] - Use of ultracentrifugation technique to increase sensitivity of RT-PCR genotyping of swine ratavirus
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