Identification of DNA repair genes in the Caulocater crescentus genome through the selection of clones sensitive to genotoxic agents (2003)
- Authors:
- USP affiliated authors: MARQUES, MARILIS DO VALLE - ICB ; MENCK, CARLOS FREDERICO MARTINS - ICB
- Unidade: ICB
- Assunto: MICROBIOLOGIA
- Language: Português
- Abstract: Caulobacter crescentus is one of the main models for studies of cellular differentiation in bacteria. Each cell division produces two different cell types: a motile - flagellum- and a stalked, sessile cell. Both differ in their structure, program of development and initiation of DNA replication. A library of approximately 5,000 mutants was constructed with a random insertion of transposon Tn5 in the genome of Caulobacter crescentus. The main objective of this work was to select possible mutants involved in DNA repair mechanisms, that assist in the protection of this bacteria the ultraviolet light (UV) and methyl metanesulfonate (MMS). A screening was carried searching for clones sensitive to these genotoxic agents. The screening was performed in genomic scale (96 clones/test) and the sensitive candidates were confirmed at least three times.Among 5,000 initial clones, we observed that 156 were sensitive to UV-light and 112 to MMS. Several had a sensitive phenotype to both treatments. These mutant clone candidates are being used to identify the genes interrupted by Tn5, through DNA sequencing. We hope this genomic strategy will allow us to have the profile of genes involved in repair of DNA damage for Caulobacter crescentus
- Imprenta:
- Publisher: Comissão de Pesquisa do ICB/USP
- Publisher place: São Paulo
- Date published: 2003
- Source:
- Título do periódico: Resumos
- Conference titles: Congresso Instituto Ciências Biomédicas, IV
-
ABNT
MARQUES, Regina Célia et al. Identification of DNA repair genes in the Caulocater crescentus genome through the selection of clones sensitive to genotoxic agents. 2003, Anais.. São Paulo: Comissão de Pesquisa do ICB/USP, 2003. . Acesso em: 18 abr. 2024. -
APA
Marques, R. C., Marques, M. do V., Menck, C. F. M., Martins-Pinheiro, M., Galhardo, R. da S., & Italiani, V. C. S. (2003). Identification of DNA repair genes in the Caulocater crescentus genome through the selection of clones sensitive to genotoxic agents. In Resumos. São Paulo: Comissão de Pesquisa do ICB/USP. -
NLM
Marques RC, Marques M do V, Menck CFM, Martins-Pinheiro M, Galhardo R da S, Italiani VCS. Identification of DNA repair genes in the Caulocater crescentus genome through the selection of clones sensitive to genotoxic agents. Resumos. 2003 ;[citado 2024 abr. 18 ] -
Vancouver
Marques RC, Marques M do V, Menck CFM, Martins-Pinheiro M, Galhardo R da S, Italiani VCS. Identification of DNA repair genes in the Caulocater crescentus genome through the selection of clones sensitive to genotoxic agents. Resumos. 2003 ;[citado 2024 abr. 18 ] - Characterization of the SOS dependent mutagenesis in Caulobacter crescentus
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- Identification of new genes belonging to the SOS network in Caulobacter crescentus
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