LPS-induce activaion of nuclear actor NF-KB in the central nervous system: attenuation by a NMDA receptor antagonist and a neural nitric oxide synthase (NNOS) inhibitor

LPS-induce activaion of nuclear actor NF-KB in the central nervous system: attenuation by a NMDA receptor antagonist and a neural nitric oxide synthase (NNOS) inhibitor (2002)

Authors:
Autor USP: SCAVONE, CRISTOFORO - ICB
Unidade: ICB
Assunto: FARMACOLOGIA
Language: Inglês
Abstract: It has been shown that inflammatory stimulus in the brain increase excitatory glutamatergic transmission especially at the level of NMDA receptors. These receptors have an important role in glutamate neurotoxicity and are in general coupled with the generation of NO through the activation of nNOS. Our aim was to test whether NMDA-NO pathway is involved in LPS activation of NF-kB in different areas of the brain. Cerebellum, hypothalamus, hippocampus, pre-frontal cortex, and striatum were isolated from male Wistar rats treated previously for 2-24 h with LPS (1mg/kg, i.v.) or saline. Gel shift assays showed that LPS activated NF-kB in all structures tested. Supershift assays indicated that the major DNA-protein complex altered by LPS corresponded to p50/p65 heterodimer. MK-801(0.3-3 mg/kg) or 7-Ni (5-25 mg/Kg), injected i.p. 20 min before LPS, partially reduced LPS effects. This reduction were not further potentiated in rats treated with both compounds, suggesting that glutamate acts on NMDA-nNOS pathway to activate NF-kB in response to LPS
Imprenta:
- Publisher: Comissão de Cultura e Extensão Universitária do ICB/USP
- Publisher place: São Paulo
- Date published: 2002
Source:
- Título: Resumos
Conference titles: Congresso Instituto Ciências Biomédicas, IV

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ABNT

GLEZER, I et al. LPS-induce activaion of nuclear actor NF-KB in the central nervous system: attenuation by a NMDA receptor antagonist and a neural nitric oxide synthase (NNOS) inhibitor. 2002, Anais.. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2002. . Acesso em: 09 jan. 2026.
APA

Glezer, I., Munhoz, C. D., Kawamoto, E. M., Marcourakis, T., Avellar, M. C. W., & Scavone, C. (2002). LPS-induce activaion of nuclear actor NF-KB in the central nervous system: attenuation by a NMDA receptor antagonist and a neural nitric oxide synthase (NNOS) inhibitor. In Resumos. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP.
NLM

Glezer I, Munhoz CD, Kawamoto EM, Marcourakis T, Avellar MCW, Scavone C. LPS-induce activaion of nuclear actor NF-KB in the central nervous system: attenuation by a NMDA receptor antagonist and a neural nitric oxide synthase (NNOS) inhibitor. Resumos. 2002 ;[citado 2026 jan. 09 ]
Vancouver

Glezer I, Munhoz CD, Kawamoto EM, Marcourakis T, Avellar MCW, Scavone C. LPS-induce activaion of nuclear actor NF-KB in the central nervous system: attenuation by a NMDA receptor antagonist and a neural nitric oxide synthase (NNOS) inhibitor. Resumos. 2002 ;[citado 2026 jan. 09 ]