LPS-induce activaion of nuclear actor NF-KB in the central nervous system: attenuation by a NMDA receptor antagonist and a neural nitric oxide synthase (NNOS) inhibitor (2002)
- Authors:
- Autor USP: SCAVONE, CRISTOFORO - ICB
- Unidade: ICB
- Assunto: FARMACOLOGIA
- Language: Inglês
- Abstract: It has been shown that inflammatory stimulus in the brain increase excitatory glutamatergic transmission especially at the level of NMDA receptors. These receptors have an important role in glutamate neurotoxicity and are in general coupled with the generation of NO through the activation of nNOS. Our aim was to test whether NMDA-NO pathway is involved in LPS activation of NF-kB in different areas of the brain. Cerebellum, hypothalamus, hippocampus, pre-frontal cortex, and striatum were isolated from male Wistar rats treated previously for 2-24 h with LPS (1mg/kg, i.v.) or saline. Gel shift assays showed that LPS activated NF-kB in all structures tested. Supershift assays indicated that the major DNA-protein complex altered by LPS corresponded to p50/p65 heterodimer. MK-801(0.3-3 mg/kg) or 7-Ni (5-25 mg/Kg), injected i.p. 20 min before LPS, partially reduced LPS effects. This reduction were not further potentiated in rats treated with both compounds, suggesting that glutamate acts on NMDA-nNOS pathway to activate NF-kB in response to LPS
- Imprenta:
- Publisher: Comissão de Cultura e Extensão Universitária do ICB/USP
- Publisher place: São Paulo
- Date published: 2002
- Source:
- Título do periódico: Resumos
- Conference titles: Congresso Instituto Ciências Biomédicas, IV
-
ABNT
GLEZER, I et al. LPS-induce activaion of nuclear actor NF-KB in the central nervous system: attenuation by a NMDA receptor antagonist and a neural nitric oxide synthase (NNOS) inhibitor. 2002, Anais.. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2002. . Acesso em: 20 abr. 2024. -
APA
Glezer, I., Munhoz, C. D., Kawamoto, E. M., Marcourakis, T., Avellar, M. C. W., & Scavone, C. (2002). LPS-induce activaion of nuclear actor NF-KB in the central nervous system: attenuation by a NMDA receptor antagonist and a neural nitric oxide synthase (NNOS) inhibitor. In Resumos. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP. -
NLM
Glezer I, Munhoz CD, Kawamoto EM, Marcourakis T, Avellar MCW, Scavone C. LPS-induce activaion of nuclear actor NF-KB in the central nervous system: attenuation by a NMDA receptor antagonist and a neural nitric oxide synthase (NNOS) inhibitor. Resumos. 2002 ;[citado 2024 abr. 20 ] -
Vancouver
Glezer I, Munhoz CD, Kawamoto EM, Marcourakis T, Avellar MCW, Scavone C. LPS-induce activaion of nuclear actor NF-KB in the central nervous system: attenuation by a NMDA receptor antagonist and a neural nitric oxide synthase (NNOS) inhibitor. Resumos. 2002 ;[citado 2024 abr. 20 ] - Cellular 'NA POT.+' Pump as a site of action for carbon monoxide and glutamate: a mechanism for long-term modulation of cellular activity
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