Cocaine activates nuclear factor NF-KB in PC 12 cells (2005)
- Authors:
- USP affiliated author: SCAVONE, CRISTOFORO - ICB
- School: ICB
- Subject: FARMACOLOGIA
- Language: Português
- Abstract: Objetivo: Drug abuse is associated with changes in brain function and neurodegenerative process, which, for some drugs, have been shown to be associated with the induction of apoptotic cell death. Cocaine is a widely used drug of abuse and a psychostimulant that acts on the central nervous system by blocking dopamine reuptake sites. NF-_B is a transcription factor which plays an important role in the control of the immunity, inflammation and apoptotic response. Our aim was to evaluate if cocaine can modulate NF-_B in PC 12 cells. Métodos e Resultados: PC 12 cells which was cultured in dishes were treated with increasing concentrations of cocaine (0,05; 0,25; 0,5; 0,75; 1,0mM) for 8 hours. Nuclear extracts were isolated and gel mobility shift assay used to measure changes in NF-_B activity. Competition studies with specific and nonspecific unlabeled double-strand oligonucleotide were also performed. Cocaine (0,75 and 1,0 mM) induced a significant induction of NF-_B in PC 12 cells. This activation was dose dependent. Conclusões: Our results suggest that cocaine induces a dose-dependent activation of NF-_B in PC 12 cells.
- Imprenta:
- Publisher: Federação de Sociedades de Biologia Experimental
- Place of publication: Águas de Lindóia, São Paulo
- Date published: 2005
- Source:
- Título do periódico: Resumos
- Conference title: Reunião Anual da Federação de Sociedades de Biologia Experimental, FeSBE
-
ABNT
LEPSCH, L. B. et al. Cocaine activates nuclear factor NF-KB in PC 12 cells. 2005, Anais.. Águas de Lindóia, São Paulo: Federação de Sociedades de Biologia Experimental, 2005. . Acesso em: 03 jul. 2022. -
APA
Lepsch, L. B., Munhoz, C. D., Kawamoto, E. M., Lima, L. S., Planeta, C. S., & Scavone, C. (2005). Cocaine activates nuclear factor NF-KB in PC 12 cells. In Resumos. Águas de Lindóia, São Paulo: Federação de Sociedades de Biologia Experimental. -
NLM
Lepsch LB, Munhoz CD, Kawamoto EM, Lima LS, Planeta CS, Scavone C. Cocaine activates nuclear factor NF-KB in PC 12 cells. Resumos. 2005 ;[citado 2022 jul. 03 ] -
Vancouver
Lepsch LB, Munhoz CD, Kawamoto EM, Lima LS, Planeta CS, Scavone C. Cocaine activates nuclear factor NF-KB in PC 12 cells. Resumos. 2005 ;[citado 2022 jul. 03 ] - Influence of the dopaminergic system, CREB, and transcription factor-κB on cocaine neurotoxicity
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