AMEBaS: automatic midline extraction and background subtraction of ratiometric fluorescence time-lapses of polarized single cells - Badain, Rafael; Damineli, Daniel S. C.; Portes, Maria Teresa; Feijó, José; Buratti, Stefano; Tortora, Giorgia; Oliveira, Hugo Neves de; César Júnior, Roberto Marcondes (Journal of Visualized Experiments, 2023)