CD8LOWTCRABLOW cells from Trypanosoma cruzi chronic infected mice produce high amounts of INF-G MRNA and display protective activity against the acute infection upon in vivo transfer (2002)
- Autor:
- Autor USP: MOSIG, JOSE MARIA ALVAREZ - ICB
- Unidade: ICB
- Assunto: IMUNOLOGIA
- Idioma: Inglês
- Resumo: CD8+ T lymphocytes expressing low levels of TCRab and CD8 molecules are present in high numbers in the spleen, liver, blood and peritoneal cavity of T. cruzi-infected chronic mice. We have analysed by RT-PCR the IFN-g production capacity of TCRabLOW CD8LOW cells or other CD8+ cell subsets from the spleen of chronic mice. The in vivo protective potential of isolated spleen TCRabLOW CD8LOW was also evaluated by transfer into acutely-infected mice. For RT-PCR experiments, CD8+ cell subsets were sorted in a FACs Vantage and the mRNA extraction carried out directly or after 6 hour in vitro incubation with plate-bound anti-CD3. For transfer experiments, CD8LOWTCRabLOW were negatively-isolated using specific antibodies conjugated with magnetic beads. CD8LOWTCRabLOW cells were injected 1 day after infection with T. cruzi (Y-strain). Control groups were injected with PBS or total chronic spleen cells. Increased amounts of mRNA were present in CD8LOWTCRabLOW cells compared to CD8HIGHCD45RCHIGH and CD8HIGHCD45RCLOW subsets or to CD8+ spleen cells from non-infected mice, both after CD3 stimuli or direct ex vivo measurement. CD8LOWTCRabLOW cells conferred in vivo protection against T. cruzi infectionPBS-injected infected mice showed increased levels of parasitemia that started by day 6 and displayed two peaks, at days 10 and 18 and 100% mortality by day 19. Mice transferred with CD8LOWTCRabLOW cells displayed a smaller parasitemia peak (day 10) that progressivelydeclined and was controlled by day 20. Nevertheless, the animals of this group succumbed by day 30 with negative parasitemias. Mice injected with unseparated chronic spleen cells showed a very small parasitemia peak by day 10 that was totally controlled by day 12, and 100% of the animals survived. Our results indicate, that in spite of receptor/correceptor down-regulation, CD8LOWTCRabLOW from chronic mice are high IFN-g producers and play an important role in in vivo control of T. cruzi parasites
- Imprenta:
- Editora: Comissão de Cultura e Extensão Universitária do ICB/USP
- Local: São Paulo
- Data de publicação: 2002
- Fonte:
- Título: Resumos
- Nome do evento: Congresso Instituto Ciências Biomédicas, IV
-
ABNT
ALVAREZ, José Maria. CD8LOWTCRABLOW cells from Trypanosoma cruzi chronic infected mice produce high amounts of INF-G MRNA and display protective activity against the acute infection upon in vivo transfer. 2002, Anais.. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2002. . Acesso em: 06 out. 2024. -
APA
Alvarez, J. M. (2002). CD8LOWTCRABLOW cells from Trypanosoma cruzi chronic infected mice produce high amounts of INF-G MRNA and display protective activity against the acute infection upon in vivo transfer. In Resumos. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP. -
NLM
Alvarez JM. CD8LOWTCRABLOW cells from Trypanosoma cruzi chronic infected mice produce high amounts of INF-G MRNA and display protective activity against the acute infection upon in vivo transfer. Resumos. 2002 ;[citado 2024 out. 06 ] -
Vancouver
Alvarez JM. CD8LOWTCRABLOW cells from Trypanosoma cruzi chronic infected mice produce high amounts of INF-G MRNA and display protective activity against the acute infection upon in vivo transfer. Resumos. 2002 ;[citado 2024 out. 06 ] - Dual aspect of the in vivo association between macrophage activation by cl-246738 and specic antibodies in experimental t. Cruzi infection
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