Characterization of Candida rugosa lipase to obtain fatty acids from the coffee oil OIL hydrolysis (2019)
- Authors:
- Autor USP: RÓS, PATRÍCIA CAROLINE MOLGERO DA - EEL
- Unidade: EEL
- Subjects: LIPASE; ENZIMAS HIDROLÍTICAS
- Keywords: Óleo do Café
- Language: Inglês
- Abstract: Enzymatic hydrolysis of oils and fats, lipolysis, is catalyzed by the enzyme lipase. The lipolysis can be used to obtain fatty acids with high added value in several areas and low energy consumption. Based on this context, the present work aimed to characterize the lipase of Candida rugosa (type VII), its biochemical (pH and temperature) and kinetic properties (km and Vmax) for its application in the hydrolysis of coffee oil. In the characterization of the pH was used 5 mL of the substrate (prepared by the oil emulsion with 7% gum arabic solution), 4 mL of sodium phosphate buffer solution (0.1 mol.L -1 , with a pH varying from 3.0 to 9.0) and enzyme preparation, 5 mg.mL-1 ). The flasks were incubated at 37 °C for 5 min in a thermostat bath with 200 rpm shaking. For the study of the effect of temperature, the same methodology of the pH effect was used, but keeping the pH of the buffer solution fixed at 7.0 and varying the temperature in the range of 25 °C to 60 °C. To verify the effect of substrate concentration on the initial rate of hydrolytic lipase reaction, the percentage of oil in the emulsion was varied from 10 to 60% (m.v -1 ). The results allowed us to determine the kinetic parameters (Km and Vmax) from the Michaelis-Menten kinetic model, following the calculation of the LineweaverBurk linearization method, thus obtaining Vmax = 4243 U.g-1 and Km = 1035mmol.L-1 . The results indicated that the ideal range optimum pH for lipase was between 7.0 and 8.0 reaching an activity 3015 U.g-1 and an optimum temperature of 40 °C, with a hydrolytic activity of 3033 U.g-1 .After the characterization stage, a complete factorial design was proposed 2 2 rotational star, in order to verify the influence of the factors: emulsifier gum arabic (0 to 3% m.m-1 ) (X1) and lipase Candida rugosa (0.1 to 0.5 m.v -1 ) (X2) in the percentage of hydrolysis of the coffee oil obtained at the end of the reaction (2 h). The results revealed conversions in fatty acids from 49% to 85.6% reached at the end of the reactions. The highest results (85.5 and 85.6%) were obtained when the concentrations of both independent variables were present at their high level, indicating a strong influence on the % hydrolyzing response variable. On the other hand, when the percentage of lipase was used at its lowest level, the lowest conversion value in free fatty acids was obtained from the hydrolysis of the coffee oil (49%).
- Imprenta:
- Publisher: Galoá Proceedings
- Publisher place: Uberlândia-MG
- Date published: 2019
- Source:
- Título: Anais do XXII Simpósio Nacional de Bioprocessos
- ISSN: 2447-2816
- Volume/Número/Paginação/Ano: p.1, 2019
-
ABNT
FERNANDES, André J e DA RÓS, Patrícia Caroline Molgero e PEREIRA, Ernandes Benedito. Characterization of Candida rugosa lipase to obtain fatty acids from the coffee oil OIL hydrolysis. 2019, Anais.. Uberlândia-MG: Galoá Proceedings, 2019. p. 1. Disponível em: https://proceedings.science/p/104141?lang=en. Acesso em: 13 fev. 2026. -
APA
Fernandes, A. J., Da Rós, P. C. M., & Pereira, E. B. (2019). Characterization of Candida rugosa lipase to obtain fatty acids from the coffee oil OIL hydrolysis. In Anais do XXII Simpósio Nacional de Bioprocessos (p. 1). Uberlândia-MG: Galoá Proceedings. Recuperado de https://proceedings.science/p/104141?lang=en -
NLM
Fernandes AJ, Da Rós PCM, Pereira EB. Characterization of Candida rugosa lipase to obtain fatty acids from the coffee oil OIL hydrolysis [Internet]. Anais do XXII Simpósio Nacional de Bioprocessos. 2019 ;1.[citado 2026 fev. 13 ] Available from: https://proceedings.science/p/104141?lang=en -
Vancouver
Fernandes AJ, Da Rós PCM, Pereira EB. Characterization of Candida rugosa lipase to obtain fatty acids from the coffee oil OIL hydrolysis [Internet]. Anais do XXII Simpósio Nacional de Bioprocessos. 2019 ;1.[citado 2026 fev. 13 ] Available from: https://proceedings.science/p/104141?lang=en - Avaliação de óleos de cianobactérias como matéria-prima lipídica para síntese de biodiesel pela rota etílica
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