3D nuclear architecture distinguishes thyroid neoplastic histotypes (2024)
- Authors:
- Autor USP: POZZO, ALINE RANGEL - Interunidades em Bioinformática
- Unidade: Interunidades em Bioinformática
- Sigla do Departamento: Bioinformática
- DOI: 10.11606/D.95.2024.tde-28052024-203350
- Subjects: SEQUENCIAMENTO GENÉTICO; MARCADOR MOLECULAR; NEOPLASIAS DA TIREOIDE
- Keywords: 3D-SIM; 3D-SIM; Diferenciação de tipos histológicos; Histotypes differentiation; Neoplasia folicular não-invasiva da tireoide com características nucleares papilífero-similares; Next generation sequencing; Papillary thyroid carcinoma; Perfil 3D de telômeros; Sequenciamento de nova geração; Telomeres molecular profile
- Language: Inglês
- Abstract: Molecular markers can be diagnostic tools to support pathological analysis in thyroid neoplasms. However, because the same markers can be observed in some benign thyroid lesions, additional approaches are necessary to differentiate thyroid tumour subtypes, prevent overtreatment and tailor specific clinical management. This applies particularly to the recently described variant of thyroid cancer referred to as non-invasive follicular thyroid neoplasm with papillary-like nuclear features (NIFTP). This variant has an estimated prevalence of 4.4% to 9.1% of all papillary thyroid carcinomas worldwide. We studied 60 thyroid lesions: 20 classical papillary thyroid carcinoma (CPTC), 20 follicular variants of PTC (FVPTC) and 20 NIFTP. We examined morphological and molecular features to identify parameters that can differentiate NIFTP from the other PTC subtypes. When blindly investigating the nuclear architecture of thyroid neoplasms, we observed that NIFTP has significantly longer telomeres than CPTC and FVPTC. Super-resolved 3D-structured illumination microscopy demonstrated that NIFTP is heterogeneous and that its nuclei contain more densely packed DNA and smaller interchromatin spaces than CPTC and FVPTC, a pattern that resembles normal thyroid tissue. These data are consistent with the observed indolent biological behaviour and favourable prognosis associated with NIFTP, which lacks BRAFV600E mutations. Of note, next-generation thyroid oncological panel sequencing could not distinguish the thyroid cancer histotypes in our study cohort. In summary, our data suggest that 3D nuclear architecture can be a powerful analytical tool to diagnose and guide the clinical management of NIFTP
- Imprenta:
- Data da defesa: 23.04.2024
- Este periódico é de acesso aberto
- Este artigo NÃO é de acesso aberto
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ABNT
POZZO, Aline Rangel. 3D nuclear architecture distinguishes thyroid neoplastic histotypes. 2024. Dissertação (Mestrado) – Universidade de São Paulo, São Paulo, 2024. Disponível em: https://www.teses.usp.br/teses/disponiveis/95/95131/tde-28052024-203350/. Acesso em: 28 jan. 2026. -
APA
Pozzo, A. R. (2024). 3D nuclear architecture distinguishes thyroid neoplastic histotypes (Dissertação (Mestrado). Universidade de São Paulo, São Paulo. Recuperado de https://www.teses.usp.br/teses/disponiveis/95/95131/tde-28052024-203350/ -
NLM
Pozzo AR. 3D nuclear architecture distinguishes thyroid neoplastic histotypes [Internet]. 2024 ;[citado 2026 jan. 28 ] Available from: https://www.teses.usp.br/teses/disponiveis/95/95131/tde-28052024-203350/ -
Vancouver
Pozzo AR. 3D nuclear architecture distinguishes thyroid neoplastic histotypes [Internet]. 2024 ;[citado 2026 jan. 28 ] Available from: https://www.teses.usp.br/teses/disponiveis/95/95131/tde-28052024-203350/
Informações sobre o DOI: 10.11606/D.95.2024.tde-28052024-203350 (Fonte: oaDOI API)
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