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Nanoparticles of PMMA-b-PDMAEMA for gene delivery - P202 (2019)

• Authors:
  • Engelmann, Karl
  • Vieira, J
  • Souza, Valdomiro Vagner de
  • Paninka, R M
  • Cuccovia, Iolanda Midea
  • Riske, Karin do Amaral
  • Arcisio-Miranda, Manoel
  • Florenzano, Fabio Herbst
  • Perez, K.
• USP affiliated authors: FLORENZANO, FÁBIO HERBST - EEL ; CUCCOVIA, IOLANDA MIDEA - IQ ; SOUZA, VALDOMIRO VAGNER DE - IQ
• Unidades: EEL; IQ
• Assunto: POLÍMEROS (MATERIAIS)
• Agências de fomento:
  • Financiamento INCT-FCx
  • Financiamento CAPES
  • Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
    Processo FAPESP: 2016/13368-4
• Language: Inglês
• Abstract: The combination of PMMA (methyl polymethacrylate), a polymer soluble in organic solvents, and the water-soluble PDMAEMA (poly-2-dimethylamine methacrylate), creates an amphiphilic copolymer (PMMA-b-PDMAEMA) with pH-dependent physico-chemical properties. The aim of this study was to prepare and characterize nanoparticles (NPs) of PMMA-b-PDMAEMA copolymers made from different PMMA to PDMAEMA molar ratio and test their potential to delivery plasmid DNA into HEK 293T cells. In this work we demonstrated the efficiency of PMMA-b-PDMAEMA NPs in cellular transfection process instead of the conventional process based on lipofectamine. The NPs were prepared using the nanoprecipitation method with some adaptations. The NPs were characterized by measuring the hydrodynamic diameter, zeta potential (ZP), X-Ray diffraction, FTIR, DSC, and TEM. To follow the transfection process using SR GSD 3D microscopy the pIRES2-GFP plasmid was bound with Rhodamine 6G-encapsulated NPs. Bare NPs presented sizes in the range of 60- 100 nm and PZ of -20 to -30 mV. The TEM and X-Ray diffraction showed spherical and solids nanoparticles, respectively. GSD microscopy showed that Rhodamine 6G-encapsulated NPs were able to pass through the HEK 293T cell plasmatic membrane and deliver the pIRES2-GFP plasmid. Phase contrast optical microscopy of POPC giant unilamellar vesicles (GUVs) showed that NPs adhered onto the lipid bilayer. The copolymer hydrophilic-hydrophobic balance was also important in GFP gene transfection since the 1:1 PMMA:PDMAEMA ratio NPs seemed to be more efficient than other copolymer studied. Furthermore, the NPs size and zeta potencial were pH sensible due to amine group present in PDMAEMA portion.
• Imprenta:
  • Publisher: ABSA
  • Publisher place: Madri
  • Date published: 2019
• Source:
  • Título: JOINT 12th EBSA congress and 10th ICBP ? IUPAP congress, July 20-24, 2019, Madrid, Spain ? Abstracts
  • Volume/Número/Paginação/Ano: p.S130-S130, 2019


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How to cite

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• ABNT

  ENGELMANN, Karl et al. Nanoparticles of PMMA-b-PDMAEMA for gene delivery - P202. 2019, Anais.. Madri: ABSA, 2019. p. S130-S130. Disponível em: https://link.springer.com/content/pdf/10.1007/s00249-019-01373-4.pdf. Acesso em: 21 out. 2024.

• APA

  Engelmann, K., Vieira, J., Souza, V. V. de, Paninka, R. M., Cuccovia, I. M., Riske, K. do A., et al. (2019). Nanoparticles of PMMA-b-PDMAEMA for gene delivery - P202. In JOINT 12th EBSA congress and 10th ICBP ? IUPAP congress, July 20-24, 2019, Madrid, Spain ? Abstracts (p. S130-S130). Madri: ABSA. Recuperado de https://link.springer.com/content/pdf/10.1007/s00249-019-01373-4.pdf

• NLM

  Engelmann K, Vieira J, Souza VV de, Paninka RM, Cuccovia IM, Riske K do A, Arcisio-Miranda M, Florenzano FH, Perez K. Nanoparticles of PMMA-b-PDMAEMA for gene delivery - P202 [Internet]. JOINT 12th EBSA congress and 10th ICBP ? IUPAP congress, July 20-24, 2019, Madrid, Spain ? Abstracts. 2019 ;S130-S130.[citado 2024 out. 21 ] Available from: https://link.springer.com/content/pdf/10.1007/s00249-019-01373-4.pdf

• Vancouver

  Engelmann K, Vieira J, Souza VV de, Paninka RM, Cuccovia IM, Riske K do A, Arcisio-Miranda M, Florenzano FH, Perez K. Nanoparticles of PMMA-b-PDMAEMA for gene delivery - P202 [Internet]. JOINT 12th EBSA congress and 10th ICBP ? IUPAP congress, July 20-24, 2019, Madrid, Spain ? Abstracts. 2019 ;S130-S130.[citado 2024 out. 21 ] Available from: https://link.springer.com/content/pdf/10.1007/s00249-019-01373-4.pdf

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