How alkaline deacetylation can enhance the building blocks obtention from sugarcane bagasse? (2019)
- Authors:
- USP affiliated authors: ROBERTO, INÊS CONCEIÇÃO - EEL ; LIMA, CLEILTON SANTOS - EEL
- Unidade: EEL
- Assunto: BIOTECNOLOGIA
- Keywords: sugarcane bagasse; deacetylation; PRETREATMENT; enzymatic saccharification
- Language: Inglês
- Abstract: Alkaline deacetylation has emerged as a promising alternative process to enhance enzymatic saccharification of sugarcane bagasse and reduce hydrolysate toxicity. In this study, we compare the combination of the sugarcane bagasse first submitted to alkaline treatment (0.4% NaOH, 70°C, 3h) followed by dilute acid pretreatment (0.5% H2SO4, 140°C, 15 min), with as a control, the bagasse only pretreated in dilute acid (0.5% H2SO4, 140°C, 15 min). Afterwards, the materials were submitted to enzymatic saccharification with Cellic® CTec 2, for 72 hours, solid-liquid ratio 1:10 (m/v), at 50°C and pH 5.0, 200 rpm of stirring and enzymatic concentration of 10 FPU/g-glucan, in triplicate. Compared to pretreated sugarcane bagasse control, the deacetylated sugarcane bagasse as feedstock is approximately 16% more digestible after pretreatment for obtention of both building blocks (glucose and xylose) achieving the conversion of 64% and 40% for cellulose and hemicellulose, respectively. It also lowered the hydrolysate toxicity, in approximately 80% for glucose and xylose utilization. Alkaline deacetylation combined to diluted acid pretreatment has demonstrate significant improvement on glucose and xylose yields during pretreatment and enzymatic saccharification, and it also reduces hydrolysate toxicity favoring the obtaining of biofuels, biochemicals and biomaterials from fermentative processes.
- Imprenta:
- Publisher place: Uberlândia-MG
- Date published: 2019
- Source:
- Título: Anais do Simpósio Nacional de Bioprocessos
- ISSN: 2447-2816
- Volume/Número/Paginação/Ano: p.1-1, 2019
-
ABNT
ANDRADE, Liliane Pires et al. How alkaline deacetylation can enhance the building blocks obtention from sugarcane bagasse? 2019, Anais.. Uberlândia-MG: Escola de Engenharia de Lorena, Universidade de São Paulo, 2019. p. 1-1. Disponível em: https://proceedings.science/p/104419. Acesso em: 08 nov. 2024. -
APA
Andrade, L. P., Lima, C. S., Roberto, I. C., Murakami, M. T., & Rocha, G. J. de M. (2019). How alkaline deacetylation can enhance the building blocks obtention from sugarcane bagasse? In Anais do Simpósio Nacional de Bioprocessos (p. 1-1). Uberlândia-MG: Escola de Engenharia de Lorena, Universidade de São Paulo. Recuperado de https://proceedings.science/p/104419 -
NLM
Andrade LP, Lima CS, Roberto IC, Murakami MT, Rocha GJ de M. How alkaline deacetylation can enhance the building blocks obtention from sugarcane bagasse? [Internet]. Anais do Simpósio Nacional de Bioprocessos. 2019 ;1-1.[citado 2024 nov. 08 ] Available from: https://proceedings.science/p/104419 -
Vancouver
Andrade LP, Lima CS, Roberto IC, Murakami MT, Rocha GJ de M. How alkaline deacetylation can enhance the building blocks obtention from sugarcane bagasse? [Internet]. Anais do Simpósio Nacional de Bioprocessos. 2019 ;1-1.[citado 2024 nov. 08 ] Available from: https://proceedings.science/p/104419 - Produção de etanol celulósico por <i>Spathaspora passalidarum</i> a partir do bagaço de cana-de-açúcar: estratégia para melhorar fermentabilidade e estudo do perfil metabólico durante fermentação de hidrolisado hemicelulósico
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