Evaluation of Enzymatic Hydrolysis of Sugarcane Bagasse Using Combination of Enzymes or Co-Substrate to Boost Lytic Polysaccharide Monooxygenases Action (2022)
- Authors:
- USP affiliated authors: SILVA, SILVIO SILVERIO DA - EEL ; SANTOS, JÚLIO CÉSAR DOS - EEL ; CUNHA, MARIA LAURA SILVA - EEL ; PRADO, CARINA ALINE - EEL
- Unidade: EEL
- DOI: 10.3390/catal12101158
- Subjects: BAGAÇOS; CANA-DE-AÇÚCAR; ENZIMAS HIDROLÍTICAS; PERÓXIDO DE HIDROGÊNIO; OLIGOSSACARÍDEOS
- Keywords: lignocellulosic biomass; sugarcane bagasse; enzymatic hydrolysis; LPMO; hydrogen peroxide; cello-oligosaccharide dehydrogenase
- Language: Inglês
- Abstract: This study evaluated innovative approaches for the enzymatic hydrolysis of lignocellulosic biomass. More specifically, assays were performed to evaluate the supplementation of the commercial cellulolytic cocktail Cellic® CTec2 (CC2) with LPMO (GcLPMO9B), H2O2, or cello-oligosaccharide dehydrogenase (CelDH) FgCelDH7C in order to boost the LPMO action and improve the saccharification efficiency of biomass into monosaccharides. The enzymatic hydrolysis was carried out using sugarcane bagasse pretreated by hydrodynamic cavitation-assisted oxidative process, 10% (w/w) solid loading, and 30 FPU CC2/g dry biomass. The results were compared in terms of sugars release and revealed an important influence of the supplementations at the initial 6 h of hydrolysis. While the addition of CelDH led to a steady increase in glucose production to reach 101.1 mg of glucose/g DM, accounting for the highest value achieved after 72 h of hydrolysis, boosting the LPMOs activity by the supplementation of pure LPMOs or the LPMO co-substrate H2O2 were also effective to improve the cellulose conversion, increasing the initial reaction rate of the hydrolysis. These results revealed that LPMOs play an important role on enzymatic hydrolysis of cellulose and boosting their action can help to improve the reaction rate and increase the hydrolysis yield. LPMOs-CelDH oxidative pairs represent a novel potent combination for use in the enzymatic hydrolysis of lignocellulose biomass. Finally, the strategies presented in this study are promising approaches for application in lignocellulosic biorefineries, especially using sugarcane bagasse as a feedstock.
- Source:
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- Licença: cc-by
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ABNT
MOYA, Eva Balaguer et al. Evaluation of Enzymatic Hydrolysis of Sugarcane Bagasse Using Combination of Enzymes or Co-Substrate to Boost Lytic Polysaccharide Monooxygenases Action. Catalysts, n. , p. 1158-1168, 2022Tradução . . Disponível em: https://doi.org/10.3390/catal12101158. Acesso em: 29 dez. 2025. -
APA
Moya, E. B., Prado, C. A., Turella, S., Silva, S. S. da, HACHEM, M. A., Dragone, G., et al. (2022). Evaluation of Enzymatic Hydrolysis of Sugarcane Bagasse Using Combination of Enzymes or Co-Substrate to Boost Lytic Polysaccharide Monooxygenases Action. Catalysts, ( ), 1158-1168. doi:10.3390/catal12101158 -
NLM
Moya EB, Prado CA, Turella S, Silva SS da, HACHEM MA, Dragone G, SANTOS JC dos, Mussato SI, Cunha MLS. Evaluation of Enzymatic Hydrolysis of Sugarcane Bagasse Using Combination of Enzymes or Co-Substrate to Boost Lytic Polysaccharide Monooxygenases Action [Internet]. Catalysts. 2022 ;( ): 1158-1168.[citado 2025 dez. 29 ] Available from: https://doi.org/10.3390/catal12101158 -
Vancouver
Moya EB, Prado CA, Turella S, Silva SS da, HACHEM MA, Dragone G, SANTOS JC dos, Mussato SI, Cunha MLS. Evaluation of Enzymatic Hydrolysis of Sugarcane Bagasse Using Combination of Enzymes or Co-Substrate to Boost Lytic Polysaccharide Monooxygenases Action [Internet]. Catalysts. 2022 ;( ): 1158-1168.[citado 2025 dez. 29 ] Available from: https://doi.org/10.3390/catal12101158 - Hydrodynamic Cavitation-Assisted Oxidative Pretreatment and Sequential Production of Ethanol and Xylitol as Innovative Approaches for Sugarcane Bagasse Biorefineries
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Informações sobre o DOI: 10.3390/catal12101158 (Fonte: oaDOI API)
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