Proteomics: a tool to investigate of composition and function of HDL in hyperlipidemia (2022)
- Authors:
- Autor USP: SILVA, AMANDA RIBEIRO MARTINS DA - IQ
- Unidade: IQ
- Sigla do Departamento: QBQ
- DOI: 10.11606/T.46.2022.tde-26082022-123137
- Subjects: PROTEÔMICA; HIPERLIPIDEMIA; LIPOPROTEÍNAS
- Keywords: Dieta rica em gordura saturada; HDL; Hiperlipidemia; Ômega-3; Proteômica quantitativa
- Agências de fomento:
- Language: Inglês
- Abstract: The inverse relationship between HDL-C (high-density lipoprotein cholesterol) and cardiovascular disease is well established. However, it is consensus that the cholesterol content present in HDL does not capture its complexity, and other metrics need to be explored. HDL is a heterogeneous, protein-enriched particle with functions going beyond lipid metabolism. In this way, its protein content seems to be attractive to investigate its behavior in the face of pathologies. Many of the proteins with important function in HDL are in low abundance (<1% of total proteins), which makes their detection challenging. Quantitative proteomics allows detecting proteins with high precision and robustness in complex matrix. However, quantitative proteomics is still poorly explored in the context of HDL. In this sense, in the second chapter of this thesis, the analytical performance of two quantitative methodologies was carefully investigated. These methods achieved adequate linearity and high precision using labeled peptides in a pool HDL, in addition to comparable ability to differentiate proteins from HDL subclasses of healthy subjects. Another bottleneck that waits for a solution in proteomics is the lack of standardization in data processing and analysis after mass spectrometry acquisition. In addition, interest in the cardioprotective properties of omega-3 is growing, but little is known about its effects on the HDL proteome. Thus, in the third chapter of this thesis, we compared five protein quantification strategies using Skyline and MaxDIA software platforms in order to investigate the HDL proteome from mice submitted to a high-fat diet supplemented or not with omega-3. MaxDIA with label-free quantification (MaxLFQ) achieved high precision to show that polyunsaturated fatty acids remodel the HDL proteome to a less inflammatory profile. Therefore, the two studies presented in this thesis begin to open newpaths for a deeper and more reliable understanding of HDL, both at the level of protein quantification by mass spectrometry and after data acquisition
- Imprenta:
- Data da defesa: 08.07.2022
- Este periódico é de acesso aberto
- Este artigo é de acesso aberto
- URL de acesso aberto
- Cor do Acesso Aberto: gold
- Licença: cc-by-nc-sa
-
ABNT
SILVA, Amanda Ribeiro Martins da. Proteomics: a tool to investigate of composition and function of HDL in hyperlipidemia. 2022. Tese (Doutorado) – Universidade de São Paulo, São Paulo, 2022. Disponível em: https://www.teses.usp.br/teses/disponiveis/46/46131/tde-26082022-123137/. Acesso em: 18 set. 2024. -
APA
Silva, A. R. M. da. (2022). Proteomics: a tool to investigate of composition and function of HDL in hyperlipidemia (Tese (Doutorado). Universidade de São Paulo, São Paulo. Recuperado de https://www.teses.usp.br/teses/disponiveis/46/46131/tde-26082022-123137/ -
NLM
Silva ARM da. Proteomics: a tool to investigate of composition and function of HDL in hyperlipidemia [Internet]. 2022 ;[citado 2024 set. 18 ] Available from: https://www.teses.usp.br/teses/disponiveis/46/46131/tde-26082022-123137/ -
Vancouver
Silva ARM da. Proteomics: a tool to investigate of composition and function of HDL in hyperlipidemia [Internet]. 2022 ;[citado 2024 set. 18 ] Available from: https://www.teses.usp.br/teses/disponiveis/46/46131/tde-26082022-123137/ - Desenvolvimento de procedimento analítico para determinação de iodato em sal de mesa utilizando sistema com multicomutação e detecção espectrofotométrica
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Informações sobre o DOI: 10.11606/T.46.2022.tde-26082022-123137 (Fonte: oaDOI API)
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