Proteomic analysis of human keratinocytes under UVA-induced stress (2021)
- Authors:
- Autor USP: VALERIO, HELLEN PAULA - IQ
- Unidade: IQ
- Sigla do Departamento: QBQ
- DOI: 10.11606/T.46.2021.tde-28042022-144138
- Subjects: RADIAÇÃO ULTRAVIOLETA; PELE; PROTEÔMICA
- Keywords: Keratinocytes; Pele; Proteômica espacial; Queratinóticos; Radiação UVA; Resposta ao estresse; Senescence; Senescência; Skin; Spatial proteomics; Stress response; UVA radiation
- Agências de fomento:
- Language: Inglês
- Abstract: The solar ultraviolet (UV) radiation that reaches the Earth is composed of 95% of UVA (320 to 400 nm) and 5% of UVB (280 to 320 nm) radiation. UVB is carcinogenic, generating potentially mutagenic DNA lesions. The solar UVA radiation also causes DNA damage, but this fact does not fully account for its biological impact. UVA is absorbed by non-DNA cellular chromophores, generating reactive oxygen species such as singlet oxygen. Knowing the proteome mediates stress responses in cells, here we investigated the cellular effects of a non-cytotoxic dose of UVA radiation, equivalent to about 20 minutes of midday sun exposure, on the proteome of human keratinocytes. Using a combination of mass spectrometry-based proteomics, bioinformatics, and conventional biochemical assays, we analyzed two aspects of UVA-induced stress: spatial remodeling of the proteome in subcellular compartments 30 minutes after stress and long-term changes in protein levels and secretion (24 hours and 7 days postirradiation). In the first part of this thesis, we quantified and assigned subcellular localization for over 3000 proteins, of which about 600 potentially redistribute upon UVA exposure. Protein redistributions were accompanied by redox modulations, mitochondrial fragmentation and DNA damage. In the second part of the work, our results showed that primary human keratinocytes enter senescence upon exposure to a single dose of UVA, mounting antioxidant and inflammatory responses. Cells under UVA-induced senescence further elicit paracrine responses in neighboring premalignant HaCaT epithelial cells via inflammatory mediators. Altogether, these results reiterate the role of UVA radiation as a potent metabolic stressor in the skin
- Imprenta:
- Data da defesa: 17.12.2021
- Este periódico é de acesso aberto
- Este artigo é de acesso aberto
- URL de acesso aberto
- Cor do Acesso Aberto: gold
- Licença: cc-by-nc-sa
-
ABNT
VALERIO, Hellen Paula. Proteomic analysis of human keratinocytes under UVA-induced stress. 2021. Tese (Doutorado) – Universidade de São Paulo, São Paulo, 2021. Disponível em: https://www.teses.usp.br/teses/disponiveis/46/46131/tde-28042022-144138/. Acesso em: 31 dez. 2025. -
APA
Valerio, H. P. (2021). Proteomic analysis of human keratinocytes under UVA-induced stress (Tese (Doutorado). Universidade de São Paulo, São Paulo. Recuperado de https://www.teses.usp.br/teses/disponiveis/46/46131/tde-28042022-144138/ -
NLM
Valerio HP. Proteomic analysis of human keratinocytes under UVA-induced stress [Internet]. 2021 ;[citado 2025 dez. 31 ] Available from: https://www.teses.usp.br/teses/disponiveis/46/46131/tde-28042022-144138/ -
Vancouver
Valerio HP. Proteomic analysis of human keratinocytes under UVA-induced stress [Internet]. 2021 ;[citado 2025 dez. 31 ] Available from: https://www.teses.usp.br/teses/disponiveis/46/46131/tde-28042022-144138/ - A single dose of Ultraviolet-A induces proteome remodeling and senescence in primary human keratinocytes
- Spatial proteomics reveals subcellular reorganization in human keratinocytes exposed to UVA light
- Outer membrane vesicles, lipidome, and biofilm formation in the endophyte enterobacter cloacae SEA01 from agave tequilana
Informações sobre o DOI: 10.11606/T.46.2021.tde-28042022-144138 (Fonte: oaDOI API)
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