Transcriptomic Profiling and Microsatellite Identification in Cobia (Rachycentron canadum), Using High-Throughput RNA Sequencing (2021)
- Authors:
- USP affiliated authors: WHITTON, RENATA GUIMARÃES MOREIRA - IB ; Cobia (Rachycentron canadum) is a marine teleost species with great productive potential worldwide. However, the genomic information currently available for this species in public databases is limited. Such lack of information hinders gene expression assessments that might bring forward novel insights into the physiology, ecology, evolution, and genetics of this potential aquaculture species. In this study, we report the first de novo transcriptome assembly of R. canadum liver, improving the availability of novel gene sequences for this species. Illumina sequencing of liver transcripts generated 1,761,965,794 raw reads, which were filtered into 1,652,319,304 high-quality reads. De novo assembly resulted in 101,789 unigenes and 163,096 isoforms, with an average length of 950.61 and 1,617.34 nt, respectively. Moreover, we found that 126,013 of these transcripts bear potentially coding sequences, and 125,993 of these elements (77.3%) correspond to functionally annotated genes found in six different databases. We also identified 701 putative ncRNA and 35,414 putative lncRNA. Interestingly, homologues for 410 of these putative lncRNAs have already been observed in previous analyses with Danio rerio, Lates calcarifer, Seriola lalandi dorsalis, Seriola dumerili, or Echeneis naucrates. Finally, we identified 7894 microsatellites related to cobia’s putative lncRNAs. Thus, the information derived from the transcriptome assembly described herein will likely assist future nutrigenomics and breeding programs involving this important fish farming species. - ; BRANCO, GIOVANA SOUZA - IB
- Unidade: IB
- DOI: 10.1007/s10126-021-10081-0/
- Subjects: EXPRESSÃO GÊNICA; AQUICULTURA; RACHYCENTRIDAE
- Agências de fomento:
- Language: Inglês
- Imprenta:
- Source:
- Título do periódico: Marine Biotechnology
- ISSN: 1436-2236
- Volume/Número/Paginação/Ano: on-line, Nov. 2021
- Este periódico é de acesso aberto
- Este artigo NÃO é de acesso aberto
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ABNT
BARBOSA, David Aciole et al. Transcriptomic Profiling and Microsatellite Identification in Cobia (Rachycentron canadum), Using High-Throughput RNA Sequencing. Marine Biotechnology, v. No 2021, 2021Tradução . . Disponível em: https://doi.org/10.1007/s10126-021-10081-0/. Acesso em: 19 abr. 2024. -
APA
Barbosa, D. A., Araújo, B. C., Branco, G. S., Simeone, A. S., Hilsdorf, A. W. S., Jabes, D. L., et al. (2021). Transcriptomic Profiling and Microsatellite Identification in Cobia (Rachycentron canadum), Using High-Throughput RNA Sequencing. Marine Biotechnology, No 2021. doi:10.1007/s10126-021-10081-0/ -
NLM
Barbosa DA, Araújo BC, Branco GS, Simeone AS, Hilsdorf AWS, Jabes DL, Nunes LR, Moreira RG, Menegidio FB. Transcriptomic Profiling and Microsatellite Identification in Cobia (Rachycentron canadum), Using High-Throughput RNA Sequencing [Internet]. Marine Biotechnology. 2021 ; No 2021[citado 2024 abr. 19 ] Available from: https://doi.org/10.1007/s10126-021-10081-0/ -
Vancouver
Barbosa DA, Araújo BC, Branco GS, Simeone AS, Hilsdorf AWS, Jabes DL, Nunes LR, Moreira RG, Menegidio FB. Transcriptomic Profiling and Microsatellite Identification in Cobia (Rachycentron canadum), Using High-Throughput RNA Sequencing [Internet]. Marine Biotechnology. 2021 ; No 2021[citado 2024 abr. 19 ] Available from: https://doi.org/10.1007/s10126-021-10081-0/ - De novo transcriptome assembly and functional annotation of cobia (Rachycentron canadum): molecular approach as a base to nutritional studies
- Effects of aquatic contaminants in female fish reproduction
- Different saturated and monounsaturated fatty acids levels in fish oil-free diets to cobia (Rachycentron canadum) juveniles: Effects in growth performance and lipid metabolism
- Hepatic postprandial molecular responses in cobia (Rachycentron canadum) juveniles
- Aspectos reprodutivos de fêmeas de piabanha Brycon insignis (Steindachner, 1877), introduzidas em ambientes alterados
- Caracterização da diferenciação celular durante a morfogênese da hipófise em Astyanax altiparanae (Garutti & Britski, 2000)
- Aspectos preliminares da biologia reprodutiva de Epinephelus marginatus (Perciformes: Serranidae) em ambiente natural e cativeiro
- Paternal exposure to aluminum, acidity, and temperature affect fatty acid seminal profile, embryonic and larval development of Astyanax altiparanae
- Efeitos do fenantreno sobre o comportamento e concentração de cortisol plasmático em Epinephelus marginatus (Perciformes: Serranidae)
- Post-feeding Molecular Responses of Cobia (Rachycentron canadum): RNA-Sequencing as a Tool to Evaluate Postprandial Effects in Hepatic Lipid Metabolism
Informações sobre o DOI: 10.1007/s10126-021-10081-0/ (Fonte: oaDOI API)
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