DNA methylation and microRNAs: gene regulation mechanisms that may interfere with apical periodontitis progression (2017)
- Authors:
- Autor USP: GARLET, GUSTAVO POMPERMAIER - FOB
- Unidade: FOB
- Subjects: PERIODONTITE PERIAPICAL; METILAÇÃO DE DNA; MICRORNAS; REGULAÇÃO GÊNICA
- Language: Inglês
- Abstract: DNA methylation is one of several epigenetic mechanisms that cells use to repress gene transcription without changing the DNA sequence. MicroRNAs (miRNAs) are short single-stranded RNA molecules that regulate gene expression in a sequence-specific manner. We hypothesized that DNA methylation and miRNAs binding events affecting immune response-related genes may contribute to apical periodontitis development. The DNA methylation profile of 22 immune response-related genes was evaluated in 49 human periapical granulomas (PG) and 10 healthy periodontal ligament tissues (PDL) using a pathway-specific real-time polymerase chain reaction array. Next-generation miRNA sequencing was performed in 9 PGs and 2 PDLs using Illumina HiSeq. Data analysis was performed using ANOVA and Pearson's correlation tests. P-values 0.05 were considered statistically significant. FAAD, INHA, and CXCL3 gene promoters showed significant hypermethylation in PG samples when compared to PDL (p<0.001). In contrast, AFT2, IL12B and IL4R showed significantly hypomethylation levels when compared with other genes (p<0.05). FAAD, CXCL3, IL12B and IL4R mRNA expression was inversely correlated to their methylation levels. A hundred and seventeen miRNAs were down-regulated and 155 were up-regulated in PG samples in comparison to PDL. Of these, miRNAs involved in AMPK and FoxO signaling pathways were down-regulated while Osteoclast differentiation and cAMP signaling pathways were up-regulated. DNA methylation and miRNAs may have a role in modulating gene expression during apical periodontitis
- Imprenta:
- Source:
- Título do periódico: Brazilian Oral Research
- Volume/Número/Paginação/Ano: 1807-3107
- Conference titles: Reunião Anual da Sociedade Brasileira de Pesquisa Odontológica - SBPqO
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ABNT
WICHNIESKI, C. et al. DNA methylation and microRNAs: gene regulation mechanisms that may interfere with apical periodontitis progression. Brazilian Oral Research. São Paulo: Faculdade de Odontologia de Bauru, Universidade de São Paulo. . Acesso em: 23 abr. 2024. , 2017 -
APA
Wichnieski, C., Letra, A., Tartari, T., Garlet, G. P., Silva, R. M., & Carneiro, E. (2017). DNA methylation and microRNAs: gene regulation mechanisms that may interfere with apical periodontitis progression. Brazilian Oral Research. São Paulo: Faculdade de Odontologia de Bauru, Universidade de São Paulo. -
NLM
Wichnieski C, Letra A, Tartari T, Garlet GP, Silva RM, Carneiro E. DNA methylation and microRNAs: gene regulation mechanisms that may interfere with apical periodontitis progression. Brazilian Oral Research. 2017 ;[citado 2024 abr. 23 ] -
Vancouver
Wichnieski C, Letra A, Tartari T, Garlet GP, Silva RM, Carneiro E. DNA methylation and microRNAs: gene regulation mechanisms that may interfere with apical periodontitis progression. Brazilian Oral Research. 2017 ;[citado 2024 abr. 23 ] - Participação de citocinas na imunomodulação da doença periodontal experimental induzida por Actinobacillus actinomycetemcomitans
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