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Expressão dos microRNAs miR-145, miR-181c, miR-199a e miR-1183 em hipocampo e sangue de pacientes com epilepsia do lobo temporal mesial (2015)

  • Authors:
  • Autor USP: LOURENÇO, LUANA GRUPIONI - FMRP
  • Unidade: FMRP
  • Sigla do Departamento: RCA
  • Subjects: EPILEPSIA DO LOBO TEMPORAL; HIPOCAMPO; BIOMARCADORES
  • Keywords: MicroRNA; Biomarkers; Hippocampus; Mesial temporal lobe epilepsy
  • Language: Português
  • Abstract: Introduction: The mesial temporal lobe epilepsy (MTLE) is the most common form of partial epilepsy and also presents significant resistance to pharmacological treatment. A major challenge for neuroscience today is the development of biomarkers that facilitate the diagnosis and prognosis for epilepsy. Recently, research has shown that microRNAs circulating in the blood are sensitive and specific biomarkers for various diseases, including CNS, they can be obtained non-invasively and represent a low cost detection method. Thus, our study aimed to analyze the expression profile of microRNAs miR-145, miR-181c, miR199a and miR-1183 in the hippocampus and leukocyte fraction of the blood of patients with MTLE and investigate whether they can assist as diagnostic and prognosis biomarkers for epilepsy. Patients and Methods: Hippocampus and blood samples of 20 patients with MTLE were used; ten with good postoperative outcome (Engel I) and ten with poor postoperative outcome (Engel III and IV), and for the control group hippocampus of necropsy and blood of healthy individuals were used. The analysis of the expression of miRNAs was performed using RQ-PCR. Results and Conclusions: The expression of miRNAs behaved differently in the hippocampus and blood of patients with MTLE when compared with the control groups. This different behavior was most evident in miR-145, which was hypo-expressed in the hippocampus and hyper-expressed in the blood. MiRNAs miR-145, miR-181c, miR199a and miR-1183 were hyper-expressed in the leukocyte fraction from the blood of patients with MTLE, and these can be potential biomarkers to aid in the diagnosis. We found no statistical differences of these miRNAs in blood and hippocampus of patients Engel I versus Engel III and IV, however, miR-1183 was progressively expressed in the blood of patients in the control groups, Engel I and Engel III and IV respectively
  • Imprenta:
  • Data da defesa: 27.10.2015
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    • ABNT

      LOURENÇO, Luana Grupioni; TIRAPELLI, Daniela Pretti da Cunha. Expressão dos microRNAs miR-145, miR-181c, miR-199a e miR-1183 em hipocampo e sangue de pacientes com epilepsia do lobo temporal mesial. 2015.Universidade de São Paulo, Ribeirão Preto, 2015. Disponível em: < http://www.teses.usp.br/teses/disponiveis/17/17137/tde-02022016-094857/ >.
    • APA

      Lourenço, L. G., & Tirapelli, D. P. da C. (2015). Expressão dos microRNAs miR-145, miR-181c, miR-199a e miR-1183 em hipocampo e sangue de pacientes com epilepsia do lobo temporal mesial. Universidade de São Paulo, Ribeirão Preto. Recuperado de http://www.teses.usp.br/teses/disponiveis/17/17137/tde-02022016-094857/
    • NLM

      Lourenço LG, Tirapelli DP da C. Expressão dos microRNAs miR-145, miR-181c, miR-199a e miR-1183 em hipocampo e sangue de pacientes com epilepsia do lobo temporal mesial [Internet]. 2015 ;Available from: http://www.teses.usp.br/teses/disponiveis/17/17137/tde-02022016-094857/
    • Vancouver

      Lourenço LG, Tirapelli DP da C. Expressão dos microRNAs miR-145, miR-181c, miR-199a e miR-1183 em hipocampo e sangue de pacientes com epilepsia do lobo temporal mesial [Internet]. 2015 ;Available from: http://www.teses.usp.br/teses/disponiveis/17/17137/tde-02022016-094857/


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