Sakuranetin derived from Baccharis retusa (Asteraceae) reduces lung vascular inflammation and remodeling induced by chronic allergic airway inflammation (2013)
- Authors:
- Autor USP: CÂMARA, NIELS OLSEN SARAIVA - ICB
- Unidade: ICB
- Subjects: IMUNOLOGIA; FLAVONÓIDES; ASMA; INFLAMAÇÃO; CAMUNDONGO; PULMÃO
- Language: Português
- Abstract: Introdução Asthma is defined as a chronic inflammatory disease that can lead to pulmonary structural alterations, including vascular and tissue inflammation and remodeling. We have previously shown that Sakuranetin was effective in counteracting airway inflammation and remodeling in an experimental asthma model. Objetivos We hypothesize that Sakuranetin (5,4’-dihydroxy-7-methoxyflavanone), a flavonoid compound derived from a plant, could also reduce lung vascular alterations in an asthma murine model. Métodos This study was approved by Ethics Committee of University of São Paulo – CAPPESQ – Protocol 338/10. Balb-C male mice (6-8 weeks) were sensitized by an ip injection of ovalbumin (OVA)+alumen on days 0 and 14 and later inhaled with OVA 1% on days 24, 26, and 28. Sakuranetin (20mg/Kg/animal-intranasal) or dexamethasone (5mg/Kg/animal-sc) was given daily, beginning on day 24. On day 29, lungs were removed and submitted to Picrosirius, Resorcin-Fuchsine and LUNA stained and to immunohistochemistry staining to visualize NFB activation and 8-isoprostane by morphometric analysis. We also quantified cytokines (RANTES, IL-5, IL-4, Eotaxin, TNF-, IFN-, IL-10 and VEGF) by BioPlex.Resultados Sakuranetin reduced eosinophils (1.37±0.39), elastic fibers content (1.69±0.08), smooth muscular layer thickness (3.72±0.14), NFB (2.21±0.27) and 8-isoprostane (6.96±0.37) induced by OVA (4.06±0.88; 2.35±0.12; 4.28±0.20; 5.17±1.19; 9.73±0.93; respectively p <0.05) in vascular wall. In lung homogenate, Sakuranetin reduced RANTES (9.38±1.39), IL-5 (5.61±1.45), Eotaxin (436.74±112.78) and VEGF (367.77±32.31) in OVA-exposed animals (24.70±5.55; 22.60±8.28; 759.39±107.10; 941.77±210.38; respectively p <0.05) compared to those treated with vehicle. Conclusão Our data demonstrated that Sakuranetin reduced vascular inflammation and remodeling in this experimental asthma model, suggesting an anti-inflammatory and antioxidant activity observed by the reduction in NFB activation, Th2 cytokines, VEGF and oxidative stress. This compound could be further investigated in order to be considered as an alternative therapeutic approach in asthma since its effects was similar of those observed by corticosteroid treatment.
- Imprenta:
- Source:
- Título: Resumos
- Conference titles: Reunião Anual da Federação de Sociedades de Biologia Experimental (FeSBE)
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ABNT
SANTANA, F. P. R. et al. Sakuranetin derived from Baccharis retusa (Asteraceae) reduces lung vascular inflammation and remodeling induced by chronic allergic airway inflammation. 2013, Anais.. Caxambu: FeSBE, 2013. . Acesso em: 07 jun. 2025. -
APA
Santana, F. P. R., Sakoda, C. P. P., Choqueta-Toledo, A., Pinheiro, N. M., Perini, A., Grecco, S., et al. (2013). Sakuranetin derived from Baccharis retusa (Asteraceae) reduces lung vascular inflammation and remodeling induced by chronic allergic airway inflammation. In Resumos. Caxambu: FeSBE. -
NLM
Santana FPR, Sakoda CPP, Choqueta-Toledo A, Pinheiro NM, Perini A, Grecco S, Tibério IFLC, Hiyane M, Lago JHG, Martins MA, Prado CM, Câmara NOS. Sakuranetin derived from Baccharis retusa (Asteraceae) reduces lung vascular inflammation and remodeling induced by chronic allergic airway inflammation. Resumos. 2013 ;[citado 2025 jun. 07 ] -
Vancouver
Santana FPR, Sakoda CPP, Choqueta-Toledo A, Pinheiro NM, Perini A, Grecco S, Tibério IFLC, Hiyane M, Lago JHG, Martins MA, Prado CM, Câmara NOS. Sakuranetin derived from Baccharis retusa (Asteraceae) reduces lung vascular inflammation and remodeling induced by chronic allergic airway inflammation. Resumos. 2013 ;[citado 2025 jun. 07 ] - A role for HO-1 in the renal function impairment inrapamucin-treated animals submitted to ischemic and reperfusion injury (IRI)
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