Interference of estradiol on the leukocyte mobilization and expression of ICAM-1 in the lung of rat subjected to intestinal ischemia/reperfusion (2013)
- Authors:
- USP affiliated authors: LIMA, WOTHAN TAVARES DE - ICB ; OLIVEIRA FILHO, RICARDO MARTINS DE - ICB
- Unidade: ICB
- Subjects: FARMACOLOGIA; RATOS WISTAR; LEUCOCITOSE ANIMAL; HORMONIOS SEXUAIS; ISQUEMIA
- Language: Português
- Abstract: Introdução Experimental evidence indicates that female sex hormones may exert protective effect on organ injury caused by trauma hemorrhagic shock model. In addition, intestinal ischemia reperfusion (IR) causes neutrophil-dependent acute lung injury. In this context female are more resistant than the male to develop adverse effects in the lung after ischemic event. Objetivos Here, in order to assess the role of estradiol on the traffic of leukocytes into the lung, we have quantified the number of circulating blood leukocytes and the expression of adhesion molecules in the lung of ovariectomized rats subjected to intestinal I/R. Métodos Intestinal I/R was induced by 45 min occlusion of the superior mesenteric artery, followed by 2 h reperfusion in female Wistar (60 days old). Ovaries removal (OVx) was carried 7 day before IR induction. OVx rats received one single dose (280 μg/kg, s.c.) of estradiol, 24 h before induction of I/R. Rats not submitted to I/R and OVx Sham-I/R rats were used as controls. The expression of ICAM-1 in the lung was performed by imunohistochemistry. Leukocytes in the lung were measured by optical microscopy after extraction in the lung digested by collagenase method whereas circulating leukocytes were quantified in blood samples.Resultados Estradiol treatment (E) of OVx-IR rats prevented the increase on the number of lung neutrophils presented by non-treated I/R rats (OVx I/R= 40.3 ± 5.1; vs OVx I/R+E= 25.2 ± 2.2 % cells/mm 2 , n=9). Estradiol treatment also reduced the number of neutrophils and monocytes in the blood of OVx-I/R rats (Neutrophils: OVx-I/R= 17,873 ± 3,289 vs OVx-I/R+E= 7,765 ± 1,693 cells/mm 3 ; n=8; Monocytes: OVx-I/R= 15,439 ± 1,464 vs OVx-I/R+E= 4,714 ± 798 cells/mm 3 ; n=8). Estradiol treatment caused a significant reduction of ICAM-1 expression (OVx-I/R= 27.3 ± 3.0 vs OVx-I/R+E= 11.4 ± 2.7 objects x10 3 /mm 2 ; n=11); Moreover, E normalized the expression of PECAM-1 (OVx I/R= 4.8 ± 1.1; OVx-I/R+E= 11.67 ± 1.6 objects x10 3 /mm 2; n=11). Conclusão Our results suggest the estradiol exerts a direct control of the lung inflammatory response caused by gut injury. Moreover, since blood leukocytes did not increase after estradiol treatment, our results could suggest that this steroid could exert some inhibitory effect on the bone marrow functional activity.
- Imprenta:
- Source:
- Título do periódico: Resumos
- Conference titles: Reunião Anual da Federação de Sociedades de Biologia Experimental (FeSBE)
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ABNT
FANTOZZI, E. T. et al. Interference of estradiol on the leukocyte mobilization and expression of ICAM-1 in the lung of rat subjected to intestinal ischemia/reperfusion. 2013, Anais.. Caxambu: FeSBE, 2013. . Acesso em: 25 abr. 2024. -
APA
Fantozzi, E. T., Breithaupth-Faloppa, A. C., Silva, F. Y. R. D., Amorim, M. B. B., Oliveira-Filho, R. M. de, Vargaftig, B. B., & Lima, W. T. de. (2013). Interference of estradiol on the leukocyte mobilization and expression of ICAM-1 in the lung of rat subjected to intestinal ischemia/reperfusion. In Resumos. Caxambu: FeSBE. -
NLM
Fantozzi ET, Breithaupth-Faloppa AC, Silva FYRD, Amorim MBB, Oliveira-Filho RM de, Vargaftig BB, Lima WT de. Interference of estradiol on the leukocyte mobilization and expression of ICAM-1 in the lung of rat subjected to intestinal ischemia/reperfusion. Resumos. 2013 ;[citado 2024 abr. 25 ] -
Vancouver
Fantozzi ET, Breithaupth-Faloppa AC, Silva FYRD, Amorim MBB, Oliveira-Filho RM de, Vargaftig BB, Lima WT de. Interference of estradiol on the leukocyte mobilization and expression of ICAM-1 in the lung of rat subjected to intestinal ischemia/reperfusion. Resumos. 2013 ;[citado 2024 abr. 25 ] - Participação do sistema linfático (SL) na lesão pulmonar causada pela isquemia/reperfusão-intestinal (I/R-i)
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