Structural transitions of translation initiation factor IF2 upon GDPNP and GDP binding in solution (2011)
- Authors:
- Autor USP: OLIVEIRA, CRISTIANO LUIS PINTO DE - IF
- Unidade: IF
- DOI: 10.1021/bi200938q
- Subjects: BIOQUÍMICA; BIOLOGIA MOLECULAR
- Language: Inglês
- Abstract: Three protein factors ensure rapid and accurate initiation of translation in bacteria. Translation initiation factor IF2 is a ribosome-dependent GTPase, which is important for correct positioning of initiator tRNA on the 30S subunit as well as ribosomal subunit joining. The solution structure of the free C-terminal part of IF2 (IF2C, comprising domains IV to VI-2) was previously determined by small-angle X-ray scattering (SAXS) [Rasmussen, L. C., et al. (2008) Biochemistry 47, 5590−5598]. In this study, adding GDP or nonhydrolyzable GTP analogue GDPNP to the protein in solution caused structural changes in the protein, in agreement with recent data determined via isothermal titration calorimetry [Hauryliuk, V., et al. (2009) J. Mol. Biol. 394, 621−626]. The p(r) function indicated an elongated conformation supported by radii of gyration of 40.1 and 44.9 Å and maximum dimensions of ∼125 and ∼150 Å for IF2C with GDPNP and GDP, respectively. The SAXS data were used to model the structure of IF2C bound to either GDPNP or GDP. The structural transitions of IF2C upon GDPNP binding and following nucleotide hydrolysis support the concept of cofactor-dependent conformational switching rather than the classical model for GTPase activity.
- Imprenta:
- Publisher place: Nashiville, TE
- Date published: 2011
- Source:
- Título: Biochemistry
- Volume/Número/Paginação/Ano: v. 50, n. 45, p. 9779-9787, 2011
- Status:
- Artigo possui versão em acesso aberto em repositório (Green Open Access)
- Versão do Documento:
- Versão submetida (Pré-print)
- Acessar versão aberta:
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ABNT
RASMUSSEN, Louise Carøe Vohlander et al. Structural transitions of translation initiation factor IF2 upon GDPNP and GDP binding in solution. Biochemistry, v. 50, n. 45, p. 9779-9787, 2011Tradução . . Disponível em: https://doi.org/10.1021/bi200938q. Acesso em: 06 maio 2026. -
APA
Rasmussen, L. C. V., Oliveira, C. L. P. de, Pedersen, J. S., Sperling-Petersen, H. U., & Mortensen, K. K. (2011). Structural transitions of translation initiation factor IF2 upon GDPNP and GDP binding in solution. Biochemistry, 50( 45), 9779-9787. doi:10.1021/bi200938q -
NLM
Rasmussen LCV, Oliveira CLP de, Pedersen JS, Sperling-Petersen HU, Mortensen KK. Structural transitions of translation initiation factor IF2 upon GDPNP and GDP binding in solution [Internet]. Biochemistry. 2011 ; 50( 45): 9779-9787.[citado 2026 maio 06 ] Available from: https://doi.org/10.1021/bi200938q -
Vancouver
Rasmussen LCV, Oliveira CLP de, Pedersen JS, Sperling-Petersen HU, Mortensen KK. Structural transitions of translation initiation factor IF2 upon GDPNP and GDP binding in solution [Internet]. Biochemistry. 2011 ; 50( 45): 9779-9787.[citado 2026 maio 06 ] Available from: https://doi.org/10.1021/bi200938q - Simulation and modeling of small angle scattering intensities
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