Intragenomic variation in the second internal transcribed spacer of the ribosomal DNA of species of the genera Culex and Lutzia (Diptera: Culicidae) (2011)
- Authors:
- USP affiliated authors: SALLUM, MARIA ANICE MUREB - FSP ; MARRELLI, MAURO TOLEDO - FSP
- Unidade: FSP
- DOI: 10.1590/S0074-02762011000100001
- Subjects: DIPTERA; CULEX (VARIABILIDADE;MORFOLOGIA); FILOGENIA (ANÁLISE GENÉTICA); TÉCNICAS E PROCEDIMENTOS DE LABORATÓRIO; BIOLOGIA MOLECULAR; VARIAÇÃO GENÉTICA; GENÉTICA MOLECULAR
- Agências de fomento:
- Language: Inglês
- Abstract: Culex is the largest genus of Culicini and includes vectors of several arboviruses and filarial worms. Many species of Culex are morphologically similar, which makes their identification difficult, particularly when using female specimens. To aid evolutionary studies and species distinction, molecular techniques are often used. Sequences of the second internal transcribed spacer (ITS2) of ribosomal DNA (rDNA) from 16 species of the genus Culex and one of Lutzia were used to assess their genomic variability and to verify their applicability in the phylogenetic analysis of the group. The distance matrix (uncorrected p-distance) that was obtained revealed intragenomic and intraspecific variation. Because of the intragenomic variability, we selected ITS2 copies for use in distance analyses based on their secondary structures. Neighbour-joining topology was obtained with an uncorrected p-distance. Despite the heterogeneity observed, individuals of the same species were grouped together and correlated with the current, morphology-based classification, thereby showing that ITS2 is an appropriate marker to be used in the taxonomy of Culex
- Imprenta:
- Publisher place: Rio de Janeiro
- Date published: 2011
- Source:
- Título do periódico: Memórias do Instituto Oswaldo Cruz
- ISSN: 0074-0276
- Volume/Número/Paginação/Ano: v. 106, n. 1, p. 1-8, Feb. 2011
- Este periódico é de acesso aberto
- Este artigo é de acesso aberto
- URL de acesso aberto
- Cor do Acesso Aberto: gold
- Licença: cc-by-nc
-
ABNT
VESGUEIRO, Fabiana Tavares et al. Intragenomic variation in the second internal transcribed spacer of the ribosomal DNA of species of the genera Culex and Lutzia (Diptera: Culicidae). Memórias do Instituto Oswaldo Cruz, v. 106, n. 1, p. 1-8, 2011Tradução . . Disponível em: https://doi.org/10.1590/S0074-02762011000100001. Acesso em: 28 mar. 2024. -
APA
Vesgueiro, F. T., Demari-Silva, B., Malafronte, R. dos S., Sallum, M. A. M., & Marrelli, M. T. (2011). Intragenomic variation in the second internal transcribed spacer of the ribosomal DNA of species of the genera Culex and Lutzia (Diptera: Culicidae). Memórias do Instituto Oswaldo Cruz, 106( 1), 1-8. doi:10.1590/S0074-02762011000100001 -
NLM
Vesgueiro FT, Demari-Silva B, Malafronte R dos S, Sallum MAM, Marrelli MT. Intragenomic variation in the second internal transcribed spacer of the ribosomal DNA of species of the genera Culex and Lutzia (Diptera: Culicidae) [Internet]. Memórias do Instituto Oswaldo Cruz. 2011 ; 106( 1): 1-8.[citado 2024 mar. 28 ] Available from: https://doi.org/10.1590/S0074-02762011000100001 -
Vancouver
Vesgueiro FT, Demari-Silva B, Malafronte R dos S, Sallum MAM, Marrelli MT. Intragenomic variation in the second internal transcribed spacer of the ribosomal DNA of species of the genera Culex and Lutzia (Diptera: Culicidae) [Internet]. Memórias do Instituto Oswaldo Cruz. 2011 ; 106( 1): 1-8.[citado 2024 mar. 28 ] Available from: https://doi.org/10.1590/S0074-02762011000100001 - Wing geometry of Culex coronator (Diptera: Culicidae) from South and Southeast Brazil
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Informações sobre o DOI: 10.1590/S0074-02762011000100001 (Fonte: oaDOI API)
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