Losartan improves insulin signaling in liver and skeletal muscle of MSG-obese rats (2008)
- Authors:
- Autor USP: CARPINELLI, ANGELO RAFAEL - ICB
- Unidade: ICB
- Assunto: FISIOLOGIA
- Language: Português
- Abstract: Introduction: Angiotensin II (Ang II) is the biological active component of renin-angiotensin system (RAS) and controls blood pressure, electrolyte balance, cell growth and vascular remodeling. In addition there is a growing body of evidence demonstrating that hypertension and insulin resistance often coexist and frequently progress to diabetes and cardiovascular disease. Objective: The aim of the present study was to evaluate the effect of RAS blockage in the early steps of insulin signaling. MSG-obese rats were obtained by neonatal monosodium glutamate (MSG) treatment. Methods: Animals animals were anesthetized with sodium thiopental and used 10-15 min later. As soon as anesthesia was assured by the loss of pedal and corneal reflexes, the abdominal cavity was opened, the portal vein was exposed and 0.2 ml of normal saline with or without insulin (2 µg) was injected. Thirty seconds after insulin injection, the liver was removed and 90 seconds later gastrocnemius was removed, minced coarsely and homogenized immediately in extraction buffer. Insulin receptor (IR), insulin receptor substrates 1 and 2 (IRS-1 and IRS-2) phosphorylations in muscle and liver were determined by immunoprecipitation/immunoblotting. Results and Discussion: Treatment with losartan (30mg/Kg/day) promoted a two-fold increase in IR, IRS1 and IRS2 phosphorylation in the liver of MSG animals. In skeletal muscle IR phosphorylation was significant increased in approximately 20%. In asame way IRS1 and IRS2 phosphorylation were approximately two-fold high compared to MSG without losartan treatment. Our results showed that the blockage of RAS by losartan treatment induced an improvement in the early steps of insulin signaling in liver and skeletal muscle of obese insulin resistant animals.
- Imprenta:
- Source:
- Título do periódico: Arquivos Brasileiros de Endocrinologia e Metabologia
- Volume/Número/Paginação/Ano: v. 52, suppl. 2, 2008
- Conference titles: Congresso Paulista de Diabetes e Metabolismo
-
ABNT
GONÇALVES, A. S. et al. Losartan improves insulin signaling in liver and skeletal muscle of MSG-obese rats. Arquivos Brasileiros de Endocrinologia e Metabologia. São Paulo: Instituto de Ciências Biomédicas, Universidade de São Paulo. . Acesso em: 04 maio 2024. , 2008 -
APA
Gonçalves, A. S., Haidar, A. A., Morgan, D., Rocha, M. S., Carpinelli, A. R., & Hirata, A. E. (2008). Losartan improves insulin signaling in liver and skeletal muscle of MSG-obese rats. Arquivos Brasileiros de Endocrinologia e Metabologia. São Paulo: Instituto de Ciências Biomédicas, Universidade de São Paulo. -
NLM
Gonçalves AS, Haidar AA, Morgan D, Rocha MS, Carpinelli AR, Hirata AE. Losartan improves insulin signaling in liver and skeletal muscle of MSG-obese rats. Arquivos Brasileiros de Endocrinologia e Metabologia. 2008 ; 52[citado 2024 maio 04 ] -
Vancouver
Gonçalves AS, Haidar AA, Morgan D, Rocha MS, Carpinelli AR, Hirata AE. Losartan improves insulin signaling in liver and skeletal muscle of MSG-obese rats. Arquivos Brasileiros de Endocrinologia e Metabologia. 2008 ; 52[citado 2024 maio 04 ] - Alterações da secreção de insulina durante a restrição alimentar
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