Methylecgonidine (AEME) actions on melatonin systhesis in the rat pineal gland. (2008)
- Authors:
- USP affiliated authors: CIPOLLA NETO, JOSE - ICB ; MARCOURAKIS, TANIA - FCF
- Unidades: ICB; FCF
- Assunto: FISIOLOGIA (HUMANO)
- Language: Português
- Abstract: Introduction: The mammalian pineal gland presents a circadian rhythm of melatonin synthesis and secretion that is imposed by noradrenaline stimulation at night. Cocaine, which is an inhibitor of noradrenaline recapture, promotes increased synthesis of melatonin in explants of pineal gland. An important metabolite of cocaine, when used in the form of "crack", is the AEME (methylecgonidine), which has effects on the central nervous system even more intense than cocaine. Objectives: The aim of this work was to study the actions of AEME on melatonin synthesis, investigating its effects in vitro, in primary cultures of pineal glands, and in vivo, analyzing the daily profile of melatonin. Methods: Pineal glands were isolated from Wistar rats and maintained in culture for 48h, in BGJb medium. Then, they were stimulated by noradrenaline (1µM) in association with AEME in different concentrations (1µM and 100µM), that was added 30 min or 48h before noradrenaline addition. Melatonin synthesis was evaluated by HPLC with electrochemical detection. Results and conclusions: AEME reduced melatonin synthesis in every concentration used, independently of incubation time (Nor 1µM = 10,56±0,61ng/gland; Nor + AEME 1µM = 7,56±0,69ng/glnd; Nor + AEME 100µM = 7,96±0,78ng/gland; Nor + AEME 100µM (48h) = 7,93±0,72ng/gland). Thus, AEME seems to inhibit melatonin synthesis in the rat pineal gland, by a yet undetermined mechanism.
- Imprenta:
- Publisher: Comissão de Cultura e Extensão Universitária do ICB/USP
- Publisher place: São Paulo
- Date published: 2008
- Source:
- Título do periódico: Resumos
- Conference titles: Congresso do Instituto de Ciências Biomédicas da Universidade de São Paulo
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ABNT
MESQUITA, L. S. M. et al. Methylecgonidine (AEME) actions on melatonin systhesis in the rat pineal gland. 2008, Anais.. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2008. . Acesso em: 19 set. 2024. -
APA
Mesquita, L. S. M., Garcia, R. C. T., Lucena, R. V. L., Abrahão, M. V., Cipolla Neto, J., Marcourakis, T., & Afeche, S. C. (2008). Methylecgonidine (AEME) actions on melatonin systhesis in the rat pineal gland. In Resumos. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP. -
NLM
Mesquita LSM, Garcia RCT, Lucena RVL, Abrahão MV, Cipolla Neto J, Marcourakis T, Afeche SC. Methylecgonidine (AEME) actions on melatonin systhesis in the rat pineal gland. Resumos. 2008 ;[citado 2024 set. 19 ] -
Vancouver
Mesquita LSM, Garcia RCT, Lucena RVL, Abrahão MV, Cipolla Neto J, Marcourakis T, Afeche SC. Methylecgonidine (AEME) actions on melatonin systhesis in the rat pineal gland. Resumos. 2008 ;[citado 2024 set. 19 ] - The muscarinic effect of anhydroecgonine methyl ester, a crack cocaine pyrolysis product, impairs melatonin synthesis in the rat pineal gland
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- Resenha sem título próprio
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