Determination of papain activity in tropical dosage forms: single laboratory validation assay (2007)
- Authors:
- USP affiliated authors: KANEKO, TELMA MARY - FCF ; MARANA, SANDRO ROBERTO - IQ ; PAOLA, MARIA VALERIA ROBLES VELASCO DE - FCF ; PINTO, CLAUDINEIA APARECIDA SALES DE OLIVEIRA - FCF
- Unidades: FCF; IQ
- Subjects: ENZIMAS PROTEOLÍTICAS; FLAVONÓIDES
- Language: Português
- Abstract: Papain is a proteolytic enzyme used in topical formulations for peeling and treatment of burns, wounds and scars. It is also used as progressive depilatory agent and as cutaneous absorption enhancer. This work was aimed at validating a spectrofluorimetic method to quantify the activity of papain in semisolid pharmaceutical dosage forms. Benzyloxycarbonyl-phenylalanyl-arginine 7-amido-4-methylcoumarin was used as substrate in assays performed in microplates. The production of 7-amido-4-methylcoumarin (7-MCA) over different periods of time was used to quantify the concentration of papain in pharmaceutical forms. This method presented a linear relationship between papain concentration and rate of 7-MCA production (R = 0.9974). Besides that, the method presented an estimated detection limit (LOD) of 0.040 USP.'mL POT. -1', an estimated quantification limit (LOQ) of 0.12 USP.'mL POT. -1', a precision (RSD%) ranging from 2.7 to 5.0 and an accuracy (E%) of 95.3 to 96.7. Components of the tested topical formulation did not interfere in the papain detection. Finally, the method is simple and fast
- Imprenta:
- Publisher place: Buenos Aires
- Date published: 2007
- Source:
- Título do periódico: Latin American Journal of Pharmacy = Acta Farmacéutica Bonaerense
- ISSN: 0326-2383
- Volume/Número/Paginação/Ano: v.26, n.5, p.771-775, 2007
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ABNT
PINTO, Claudinéia Aparecida Sales de Oliveira; GREEN, Denise Helena; BABY, André Rolim; et al. Determination of papain activity in tropical dosage forms: single laboratory validation assay. Latin American Journal of Pharmacy = Acta Farmacéutica Bonaerense, Buenos Aires, v. 26, n. 5, p. 771-775, 2007. -
APA
Pinto, C. A. S. de O., Green, D. H., Baby, A. R., Ruas, G. W., Kaneko, T. M., Marana, S. R., & Velasco, M. V. R. (2007). Determination of papain activity in tropical dosage forms: single laboratory validation assay. Latin American Journal of Pharmacy = Acta Farmacéutica Bonaerense, 26( 5), 771-775. -
NLM
Pinto CAS de O, Green DH, Baby AR, Ruas GW, Kaneko TM, Marana SR, Velasco MVR. Determination of papain activity in tropical dosage forms: single laboratory validation assay. Latin American Journal of Pharmacy = Acta Farmacéutica Bonaerense. 2007 ;26( 5): 771-775. -
Vancouver
Pinto CAS de O, Green DH, Baby AR, Ruas GW, Kaneko TM, Marana SR, Velasco MVR. Determination of papain activity in tropical dosage forms: single laboratory validation assay. Latin American Journal of Pharmacy = Acta Farmacéutica Bonaerense. 2007 ;26( 5): 771-775. - Caracterização física, físico-química e química da turfa, lama sulfurosa e fango visando sua aplicação cosmética
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