The transcription of the gene for iso-orotate decarboxylase (IDCase), an enzyme of the thymidine salvage pathway, is downregulated in the 'preg POT.c' mutant strain of Neurospora crassa grown under phosphate starvation (2007)
- Authors:
- USP affiliated authors: ROSSI, NILCE MARIA MARTINEZ - FMRP ; ROSSI FILHO, ANTONIO - FMRP
- School: FMRP
- DOI: 10.1139/w07-064
- Subjects: FUNGOS; METABOLISMO ENERGÉTICO
- Language: Inglês
- Abstract: Le gène preg code une protéine de type cycline impliquée dans la dé-répression des nucléases et des phosphatases qui capturent le phosphate de lenvironnement. Afin de mieux comprendre le rôle régulateur du produit du gène preg, une DDRT-PCR (differential display reverse transcriptase- polymerase chain reaction) a été utilisée pour isoler les transcrits exprimés de façon différentielle chez la souche mutante preg POT. c de la moisissure Neurospora crassa cultivée en condition de privation en phosphate à pH 7,8. Deux transcrits dont lexpression différentielle a été confirmée par buvardage Northern étaient régulés négativement chez une souche de N. crassa portant une mutation de perte de fonction du gène preg (allèle preg POT. c). Ces transcrits correspondaient à des gènes codant des enzymes impliquées dans la voie de sauvegarde de la thymidine (iso-orotate décarboxylase) et de la biosynthèse de la coenzyme Q (ubiquinone C-méthyltransférase). Ces observations pourraient nous permettre de mieux comprendre les événements moléculaires impliqués de la perception du phosphore chez N. crassa
- Imprenta:
- Source:
- Título do periódico: Canadian Journal of Microbiology
- ISSN: 0008-4166
- Volume/Número/Paginação/Ano: v. 53, n. 8, p. 1011-1015, 2007
- Este periódico é de assinatura
- Este artigo NÃO é de acesso aberto
- Cor do Acesso Aberto: closed
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ABNT
LEAL, Juliana et al. The transcription of the gene for iso-orotate decarboxylase (IDCase), an enzyme of the thymidine salvage pathway, is downregulated in the 'preg POT.c' mutant strain of Neurospora crassa grown under phosphate starvation. Canadian Journal of Microbiology, v. 53, n. 8, p. 1011-1015, 2007Tradução . . Acesso em: 05 jul. 2022. -
APA
Leal, J., Squina, F. M., Martinez-Rossi, N. M., & Rossi, A. (2007). The transcription of the gene for iso-orotate decarboxylase (IDCase), an enzyme of the thymidine salvage pathway, is downregulated in the 'preg POT.c' mutant strain of Neurospora crassa grown under phosphate starvation. Canadian Journal of Microbiology, 53( 8), 1011-1015. doi:10.1139/w07-064 -
NLM
Leal J, Squina FM, Martinez-Rossi NM, Rossi A. The transcription of the gene for iso-orotate decarboxylase (IDCase), an enzyme of the thymidine salvage pathway, is downregulated in the 'preg POT.c' mutant strain of Neurospora crassa grown under phosphate starvation. Canadian Journal of Microbiology. 2007 ; 53( 8): 1011-1015.[citado 2022 jul. 05 ] -
Vancouver
Leal J, Squina FM, Martinez-Rossi NM, Rossi A. The transcription of the gene for iso-orotate decarboxylase (IDCase), an enzyme of the thymidine salvage pathway, is downregulated in the 'preg POT.c' mutant strain of Neurospora crassa grown under phosphate starvation. Canadian Journal of Microbiology. 2007 ; 53( 8): 1011-1015.[citado 2022 jul. 05 ] - The transcriptional modulators nuc-1 and pacC-1 from N.crassa are required for the expression of a heat shock-inducible gene of the hsp70 family
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Informações sobre o DOI: 10.1139/w07-064 (Fonte: oaDOI API)
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