Detection and identification of Leishmania species in field-captured phlebotomine sandflies based on mini-exon gene PCR (2006)
- Authors:
- USP affiliated authors: GALATI, EUNICE APARECIDA BIANCHI - FSP ; MALAFRONTE, ROSELY DOS SANTOS - FM ; JUNIOR, HEITOR FRANCO DE ANDRADE - FM
- Unidades: FSP; FM
- DOI: 10.1016/j.actatropica.2006.08.009
- Subjects: INFECÇÕES POR MASTIGOFOROS (DIAGNÓSTICO;TRANSMISSÃO); INSETOS VETORES; LEISHMANIA; DIPTERA
- Language: Inglês
- Abstract: Leishmaniasis is one of the most diverse and complex of all vector-borne diseases. Because it involves several overlapping species and sandfly vectors, the disease has a complex ecology and epidemiology. Adequate therapy and follow-up depend on parasitological diagnosis, but classical methods present several constraints when identifying species. We describe a polymerase chain reaction (PCR) which uses primers designed from mini-exon repetitive sequences that are specific for subgenus Leishmania Viannia (PV), as well as sequences with specificity for genus (PG) that can distinguish between Leishmania species from other insect flagellates with minor differences in PCR products. For standardization, these PCR were tested in experimentally infected sandflies, and Leishmania infection in these insects was successfully confirmed. This methodology identified a 3.9 percent infection rate in field-captured phlebotomine sandflies from an endemic region in Brazil. Natural infection by Leishmania species was identified in three samples of Lutzomyia longipalpis, of which two were Leishmania (L.) chagasi and one Leishmania (L.) amazonensis. Irrespective of specific epidemiological conclusions, the method used in this study was able to identify Leishmania infections both in experimentally infected and field-captured phlebotomine sandflies, and could be a useful tool in epidemiological studies and strategic planning for the control of human leishmaniasis
- Imprenta:
- Source:
- Título: Acta tropica
- ISSN: 0001-706X
- Volume/Número/Paginação/Ano: v. 99, n. 2-3, p. 252-259, 2006
- Este periódico é de assinatura
- Este artigo NÃO é de acesso aberto
- Cor do Acesso Aberto: closed
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ABNT
PAIVA, B. R. et al. Detection and identification of Leishmania species in field-captured phlebotomine sandflies based on mini-exon gene PCR. Acta tropica, v. 99, n. 2-3, p. 252-259, 2006Tradução . . Disponível em: https://doi.org/10.1016/j.actatropica.2006.08.009. Acesso em: 02 out. 2024. -
APA
Paiva, B. R., Secundino, N. F. C., Nascimento, J. C., Pimenta, P. F. P., Andrade Júnior, H. F. de, Galati, E. A. B., et al. (2006). Detection and identification of Leishmania species in field-captured phlebotomine sandflies based on mini-exon gene PCR. Acta tropica, 99( 2-3), 252-259. doi:10.1016/j.actatropica.2006.08.009 -
NLM
Paiva BR, Secundino NFC, Nascimento JC, Pimenta PFP, Andrade Júnior HF de, Galati EAB, Andrade Júnior HF de, Malafronte RS. Detection and identification of Leishmania species in field-captured phlebotomine sandflies based on mini-exon gene PCR [Internet]. Acta tropica. 2006 ; 99( 2-3): 252-259.[citado 2024 out. 02 ] Available from: https://doi.org/10.1016/j.actatropica.2006.08.009 -
Vancouver
Paiva BR, Secundino NFC, Nascimento JC, Pimenta PFP, Andrade Júnior HF de, Galati EAB, Andrade Júnior HF de, Malafronte RS. Detection and identification of Leishmania species in field-captured phlebotomine sandflies based on mini-exon gene PCR [Internet]. Acta tropica. 2006 ; 99( 2-3): 252-259.[citado 2024 out. 02 ] Available from: https://doi.org/10.1016/j.actatropica.2006.08.009 - Padronização de condições para detecção de DNA de Leishmania spp. em flebotomíneos (Diptera, Psychodidae) pela reação em cadeia da polimerase
- Natural infection of phlebotomines (Diptera: Psychodidae) in a visceral-leishmaniasis focus in Mato Grosso do Sul, Brazil
- Malária assintomática em populações residentes nos parques estaduais Petar e Intervales, na mata Atlântica
- Species-specific identification of Leishmania in naturally infected sand flies captured in Mato Grosso do Sul State, Brazil
- Estudo soroepidemiológico em dois parques estaduais localizados na mata Atlântica no estado de São Paulo
- Canine visceral leishmaniasis in the metropolitan area of São Paulo: pintomyia fischeri as potential vector of leishmania infantum
- Single step polymerase chain reaction (PCR) for the diagnosis of the Leishmania (viannia) subgenus
- Padronização da técnica de PCR para identificação de espécies de leishmanias em insetos experimentalmente infectados e sua aplicabilidade em estudos de campo
- Nyssomyia intermedia (Lutz & Neiva) and Nyssomyia neivai (Pinto) (Diptera, Psychodidae, Phlebotominae) in a sympatric area: seasonal and nocturnal hourly rhythm in black and white modified Shannon traps
- Utilização de antigenos de malária de roedores para detecção sorológica de infecção malárica pregressa: resultados preliminares
Informações sobre o DOI: 10.1016/j.actatropica.2006.08.009 (Fonte: oaDOI API)
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