Supression of asthma-like responses by recombinant BCG is not associated with increased influx of regulatory T cells to the lung in a murine model of allergic inflammation (2006)
- Authors:
- Autor USP: RUSSO, MOMTCHILO - ICB
- Unidade: ICB
- Assunto: IMUNOLOGIA
- Language: Inglês
- Abstract: BACKGROUND: We have previously shown that intranasal (in) inoculation of recombinant BCG (rBCG) expressing the subunit 1 of pertussis toxin (S1PT) was able to suppress asthma-like responses when administrated before, during or after allergen (OVA) sensitization. For instance, in rBCG-S1PT administration decreased airway eosinophilia, airway hyperreactivity and type-2 cytokine secretion. AIM: Here, we determined whether suppression of asthma-like responses by rBCG-S1PT is associated with regulatory T cells (Treg) as has been suggested by other studies using Mycobacteria. METHODS: BALB/c mice were subcutaneously immunized at days 0 and 7 with OVA/Alum and challenged with 50ug of OVA by in route at days 14 and 21 (allergic group). rBCG-S1PT group was inoculated with 106 CFU of rBCG-S1PT by intranasal instillation after OVA sensitization and after 30 days was challenged with in OVA. Control group received only PBS. The experiments were performed 24 h after the last OVA challenge. Lung and Bronchoalveolar lavage (BAL) cells were collected and FACS analysis performed for the following cell markers: CD4, CD8, CD25, CD45RB, FoxP3 and IFN- .RESULTS Surprisingly, we found that CD4+CD25+CD45RBlow T cells in the BAL and lung were higher in allergic mice than in the control or the rBCG-S1PT group. Moreover, CD4+CD25+FoxP3+ T cells in the BAL were remarkably reduced in the rBCG-S1PT group (6,4%), when compared to the allergicgroup (11,1%). We also observed an increased proportion of IFN- -producing CD4+ T cells in the lungs of the rBCG-S1PT group (0,74%), when compared to the allergic (0,28%) and control (0,034%) groups. CONCLUSION: rBCG-S1PT administration decreases asthma-like responses and this suppression is not due to the recruitment of regulatory CD4+CD25+ T cells to the pulmonary compartment. IFN- production by lung CD4+ T cells appears to play an important role in down modulation of allergic responses in this model.
- Imprenta:
- Source:
- Título: Abstracts
- Conference titles: Meeting of the Brazilian Society for Immunology
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ABNT
CHRIST, A P G et al. Supression of asthma-like responses by recombinant BCG is not associated with increased influx of regulatory T cells to the lung in a murine model of allergic inflammation. 2006, Anais.. São Paulo: Instituto de Ciências Biomédicas, Universidade de São Paulo, 2006. . Acesso em: 28 dez. 2025. -
APA
Christ, A. P. G., Rodriguez, D., Keller, A. C., Leite, L. C. C., & Russo, M. (2006). Supression of asthma-like responses by recombinant BCG is not associated with increased influx of regulatory T cells to the lung in a murine model of allergic inflammation. In Abstracts. São Paulo: Instituto de Ciências Biomédicas, Universidade de São Paulo. -
NLM
Christ APG, Rodriguez D, Keller AC, Leite LCC, Russo M. Supression of asthma-like responses by recombinant BCG is not associated with increased influx of regulatory T cells to the lung in a murine model of allergic inflammation. Abstracts. 2006 ;[citado 2025 dez. 28 ] -
Vancouver
Christ APG, Rodriguez D, Keller AC, Leite LCC, Russo M. Supression of asthma-like responses by recombinant BCG is not associated with increased influx of regulatory T cells to the lung in a murine model of allergic inflammation. Abstracts. 2006 ;[citado 2025 dez. 28 ] - Toll-like receptor 4 agonist suppresses asthma-like responses via nitric oxide synthase 2 activity
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