Putative immunosupressive tole of phosphatidylserine-exposing vesicles from B16F10 cells in malignant melanoma establishment (2006)
- USP affiliated authors: BARCINSKI, MARCELLO ANDRE - ICB ; CHAMMAS, ROGER - FM
- Unidades: ICB; FM
- Assunto: PARASITOLOGIA
- Language: Inglês
- Abstract: Introduction and objectives: It is known that phosphatidylserine (PS) exposure on cellular membranes, vesicles and liposomes stimulates anti-inflammatory responses, including the release of TGF- 1, a mediator of malignant progression. Preliminary results of our group demonstrated that B16F10 melanoma cells produce large quantities of vesicles in vitro and both, cells and vesicles, are able to up-regulate TGF- 1 production by macrophages. These data, together with the fact that tumor vesiculation has been related to the acquisition of metastatic capability and to evasion from immune surveillance, led us to investigate the involvement of PS exposure and vesicles production on the establishment of malignant melanoma. Methods and results: Now we show that tumoral microparticles carry tumor surface molecules, such as PS (data observed by FACs analysis, fluorescence microscopy and transmission electron microscopy), as well as the ganglioside GM3, involved on melanoma B16 progression.In in vivo assays, we observed that sensitization with vesicles prior to B16F10 intravenous innoculum increases the number of pulmonary tumor nodules in C57BL/6 mice (syngeneic hosts). This increase was not observed in the animals pre-sensitized with annexin V-treated vesicles or with those purified from cell cultures with low ganglioside exposure, which suggests the involvement of both, ganglioside and PS, on the modulation of the antitumoralautologous response by these microparticles. Furthermore, co-innoculum of melanoma cells and vesicles allowed tumor development in BALB/c mice that would normally sustain an efficient antitumoral response. Conclusions: Altogether, our data suggest a potential role for melanoma vesicles in tumor establishment, possibly by the negative regulation of anti-tumoral immune responses.
- Título do periódico: Abstracts
- Conference titles: Meeting of the Brazilian Society for Immunology
ABNTLIMA, L G; CHAMMAS, R; BARCINSKI, Marcello André; MOREIRA, M E C. Putative immunosupressive tole of phosphatidylserine-exposing vesicles from B16F10 cells in malignant melanoma establishment. Anais.. São Paulo: [s.n.], 2006.
APALima, L. G., Chammas, R., Barcinski, M. A., & Moreira, M. E. C. (2006). Putative immunosupressive tole of phosphatidylserine-exposing vesicles from B16F10 cells in malignant melanoma establishment. In Abstracts. São Paulo.
NLMLima LG, Chammas R, Barcinski MA, Moreira MEC. Putative immunosupressive tole of phosphatidylserine-exposing vesicles from B16F10 cells in malignant melanoma establishment. Abstracts. 2006 ;
VancouverLima LG, Chammas R, Barcinski MA, Moreira MEC. Putative immunosupressive tole of phosphatidylserine-exposing vesicles from B16F10 cells in malignant melanoma establishment. Abstracts. 2006 ;
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