ERK activity in B-1 cells is important for increasing the metastatic potential of murine melanoma cells (2006)
- Autor USP: MARIANO, MARIO - ICB
- Unidade: ICB
- Assunto: IMUNOLOGIA
- Language: Inglês
- Abstract: Introduction and Objectives: B-1 cells are the most prevalent linage of B cells in the peritoneal and pleural cavities of mice (J. Exp. Med. 161: 1554-1568, 1985). Previous studies in our group demonstrated that co-cultivation of B-1 cells with B16F10 murine melanoma cells increases the metastatic potential of the later (Melanoma Res. 13(1): 11-8, 2003). However, the mechanism underlying how B1 cells influence the intracellular signaling pathways involved in this effect has not yet been addressed. Among the signaling pathways, activation of the extracellular signal-regulated kinase (ERK) and protein kinase C (PKC) have been correlated with metastatic spread in several cancer types including melanoma (Cancer Res. 63(4): 756-9, 2003; Cancer Metastasis Rev. 24(2): 287-300, 2005). Therefore, the aim of this work was to investigate whether B1 cells increases the metastatic potential of B16F10 melanoma cells by modulating the activation of ERK and/or PKC in these cells.Methods and Results: Protein expression and phosphorylation status of PKC and ERK were evaluate in lysates of melanoma cells co-cultured or not with B-1 cells in the presence or absence of pharmacological inhibitors of either PKC (Gö6976) or ERK (PD98059) by western blotting. The biological effects of these inhibitors were studied by experimental metastases assay in vivo. We showed that: 1- ERK is constitutively activated in melanoma cells and is up-regulated aftercontact with B-1 cells. 2- Treatment with either Gö6976 or PD98059 during co-cultivation of B-1 cells with melanoma cells inhibited the increased metastatic potential induced by B-1 cells. 3- Previous treatment of melanoma cells with Gö6976 but not with PD98059 before co-cultivation of these cells with B-1 cells inhibited this effect. 4- Inhibition of ERK activity in B1 cells prevents their capacity to increase the metastatic potential of melanoma cells. Conclusion: ERK activity in B-1 cells is important for increasing the metastatic potential of murine melanoma cells.
- Título do periódico: Abstracts
- Conference titles: Meeting of the Brazilian Society for Immunology
ABNTPÉREZ, E C; MACHADO JUNIOR, J; ALIPERTI, F; MARIANO, Mario; LOPES, J D. ERK activity in B-1 cells is important for increasing the metastatic potential of murine melanoma cells. Anais.. São Paulo: [s.n.], 2006.
APAPérez, E. C., Machado Junior, J., Aliperti, F., Mariano, M., & Lopes, J. D. (2006). ERK activity in B-1 cells is important for increasing the metastatic potential of murine melanoma cells. In Abstracts. São Paulo.
NLMPérez EC, Machado Junior J, Aliperti F, Mariano M, Lopes JD. ERK activity in B-1 cells is important for increasing the metastatic potential of murine melanoma cells. Abstracts. 2006 ;
VancouverPérez EC, Machado Junior J, Aliperti F, Mariano M, Lopes JD. ERK activity in B-1 cells is important for increasing the metastatic potential of murine melanoma cells. Abstracts. 2006 ;
- Inibicao de atividades do veneno de bothrops jararaca por cininogenio isolado do plasma de serpentes da mesma especie
- Fagocitose de neutrofilos e seus granulos por macrofagos inflamatorios de camundongos das linhagens balb / c e c57bl/6
- Effect of mycobacterium leprae on the activity of peritoneal macrophages of mice from the a / 'SN' and balb / c strain influence of bacterial delipidation
- Macrofago modulador da resposta inflamatoria induzida por carragenina em camundongos: estudo da capacidade de inibicao do spreading de macrofagos ativados
- Caracterizacao e purificacao do fator secretado pelo macrofago modulador
- Crotalus durissus terrificus venom inhibits some macrophage functions and is endowed with antiinflammatory properties
- Participacao do neutrofilo no controle da dor inflamatoria
- Reconhecimento de proteinas do fator desativador de macrofagos por elisa
- Morphological aspects of free floating schistosoma egg granulomata in the peritoneal cavity of infected mice
- Morphological aspects of free floating Schistosoma egg granulomata in the peritoneal cavity of infected mice
How to cite
A citação é gerada automaticamente e pode não estar totalmente de acordo com as normas