The role of nitric oxide and hydrogen peroxide in the infection of peritoneal macrophages by T. cruzi parasites of the sylvio X10/4 and Y strains (2006)
- USP affiliated authors: LIMA, MARIA REGINA D'IMPERIO - ICB ; MOSIG, JOSE MARIA ALVAREZ - ICB ; NEGRO, SONIA JANCAR - ICB
- Unidades: ICB; ICB; ICB
- Subjects: IMUNOLOGIA
- Language: Inglês
- Abstract: Introduction and Objectives: The role of Nitric Oxide (NO) for the microbicide activity of macrophages against T. cruzi parasites has been previously demonstrated. Besides NO, macrophages produce Hydrogen Peroxide and other oxygen radical intermediates (ROIs), a process that can be increased by stimulation with IFN- . Although T. cruzi has been shown to be sensible to oxigen radicals (Mol.Biochem.Parasitol. 133:37 - 43, 2004), demonstration of the contribution of ROIs to the macrophage tripanocide activity awaits formal proof. The objective of this work is to analyze the contribution of NO and Hydrogen Peroxide to the intracellular control of T. cruzi of the Sylvio X10/4 and Y strains in peritoneal macrophages (MPs). Methods and Results: The elements that affect the resistance/susceptibility of macrophages to T. cruzi infection were evaluated by capture ELISA, measuring the parasite antigen level of lysates obtained from cultures of MPs infected in vitro with trypomastigotes. Y strain parasites were notably more resistant to the intracellular MPs milieu than Sylvio X10/4 parasites. A direct correlation was observed between MPs' resistance to the two parasites and the amount of NO and Hydrogen Peroxide produced by infected MPs.For both T. cruzi strains, pre-treatment with IFN- increased MPs resistance and the amount of secreted NO and Hydrogen Peroxide. Treatment of MPs with Apocynin, an inhibitor of membrane NAD(P)Hoxidase, resulted in complete abolishment of Hydrogen Peroxide production and in significant increases in the proliferation of parasites of both strains, an effect that did not result from interference in the production of NO. The effect of Apocynin increasing T. cruzi proliferation was also observed with MPs from iNOS-KO mice, a result that indicates that the protective effect of ROIs is not due exclusively to Peroxynitrite induction. The results obtained by capture ELISA were confirmed by examination of infected macrophage slides under the microscope. Conclusion: The present report demonstrates, for the first time, that, besides NO, ROIs contribute to the intracellular killing of T. cruzi by infected macrophages.
- Título do periódico: Abstracts
- Conference title: Meeting of the Brazilian Society for Immunology
ABNTMARTINS SANTOS, R; NUÑES, L N A; MARINHO, C R F; et al. The role of nitric oxide and hydrogen peroxide in the infection of peritoneal macrophages by T. cruzi parasites of the sylvio X10/4 and Y strains. Anais.. São Paulo: [s.n.], 2006.
APAMartins Santos, R., Nuñes, L. N. A., Marinho, C. R. F., Bastos, K. R. B., Sardinha, L. R., Serezani, C. H., et al. (2006). The role of nitric oxide and hydrogen peroxide in the infection of peritoneal macrophages by T. cruzi parasites of the sylvio X10/4 and Y strains. In Abstracts. São Paulo.
NLMMartins Santos R, Nuñes LNA, Marinho CRF, Bastos KRB, Sardinha LR, Serezani CH, Jancar S, D'Império Lima MR, Alvarez JM. The role of nitric oxide and hydrogen peroxide in the infection of peritoneal macrophages by T. cruzi parasites of the sylvio X10/4 and Y strains. Abstracts. 2006 ;
VancouverMartins Santos R, Nuñes LNA, Marinho CRF, Bastos KRB, Sardinha LR, Serezani CH, Jancar S, D'Império Lima MR, Alvarez JM. The role of nitric oxide and hydrogen peroxide in the infection of peritoneal macrophages by T. cruzi parasites of the sylvio X10/4 and Y strains. Abstracts. 2006 ;
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