Products of the lipoxygenase pathway of arachidonic acid metabolism play a role in the control of parasite burden, modulation of nitric oxide production and contribute to the oxidative stress during Trypanosoma cruzi infection (2006)
- Authors:
- Autor USP: RIZZO, LUIZ VICENTE - ICB
- Unidade: ICB
- Assunto: IMUNOLOGIA
- Language: Inglês
- Abstract: Introduction and Objectives: Recently, we showed that nitric oxide (NO) produced during Trypanosoma cruzi infection increases erythrocyte oxidative stress, but, is not necessary for the development of anemia in the acute phase of infection in mice (FEMS Immunol.Med.Microbiol., 41:69-7,2004). Here, we investigated the effects of inhibition of leukotrienes on control of parasite burden and erythrocyte oxidative stressing during T.cruzi infection in C56BL/6 and C57BL/6 iNOS-/- (KO to inducible nitric oxide synthase). Methods and Results: We assessed oxidative stress in erythrocytes from mice infected treated or not with nordihydroguaiaretic acid (NDGA, 50 mg/Kg/mice) by measuring oxyhaemoglobin and haemoglobin derivatives (i.e., methaemoglobin and hemichrome), oxygen uptake and induction time (T ind) following treatment with t-butyl hydroperoxide (t-BHT).We found that the increase of erythrocyte oxidative stress an acute infection of mice with T. cruzi was inhibited by lipoxygenase inhibitor (NDGA) (Induction time:139,7 ± 6,9s vs 171,3 ± 8,3s P< 0,001);(Oxygen uptake: 50,8 ± 2,7 nMOL/mg prot. vs 40,4 ± 3,0 nMOL/mg prot. P < 0,001). Treatment with NDGA significantly increased blood and cardiac parasitism. The heart tissue from treated mice had four-fold more parasites nests than those of non-treated mice on day 12-post-infection (4,1 ± 0,8 vs 1,1 ± 0,15, P < 0.05, Bonferroni multiple comparisons test. The NDGa treatment partially decreasedthe NO production by activated macrophages (45,8 ± 1,5 vs 38,0 ± 1,7, P < 0,001). Conclusion: Taken together, these data suggest that leukotrienes play an essential role in controlling parasite burdens in blood and heart, as well as, in the regulation of NO production from activated macrophages during acute phase of experimental infection with T.cruzi.
- Imprenta:
- Source:
- Título: Abstracts
- Conference titles: Meeting of the Brazilian Society for Immunology
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ABNT
BORGES, C L et al. Products of the lipoxygenase pathway of arachidonic acid metabolism play a role in the control of parasite burden, modulation of nitric oxide production and contribute to the oxidative stress during Trypanosoma cruzi infection. 2006, Anais.. São Paulo: Instituto de Ciências Biomédicas, Universidade de São Paulo, 2006. . Acesso em: 22 fev. 2026. -
APA
Borges, C. L., Tatakihara, V. L. H., Malvezi, A. D., Graça-de-Souza, V. K., Yamada-Ogatta, S. F., Rizzo, L. V., et al. (2006). Products of the lipoxygenase pathway of arachidonic acid metabolism play a role in the control of parasite burden, modulation of nitric oxide production and contribute to the oxidative stress during Trypanosoma cruzi infection. In Abstracts. São Paulo: Instituto de Ciências Biomédicas, Universidade de São Paulo. -
NLM
Borges CL, Tatakihara VLH, Malvezi AD, Graça-de-Souza VK, Yamada-Ogatta SF, Rizzo LV, Cecchini R, Pinge-Filho P. Products of the lipoxygenase pathway of arachidonic acid metabolism play a role in the control of parasite burden, modulation of nitric oxide production and contribute to the oxidative stress during Trypanosoma cruzi infection. Abstracts. 2006 ;[citado 2026 fev. 22 ] -
Vancouver
Borges CL, Tatakihara VLH, Malvezi AD, Graça-de-Souza VK, Yamada-Ogatta SF, Rizzo LV, Cecchini R, Pinge-Filho P. Products of the lipoxygenase pathway of arachidonic acid metabolism play a role in the control of parasite burden, modulation of nitric oxide production and contribute to the oxidative stress during Trypanosoma cruzi infection. Abstracts. 2006 ;[citado 2026 fev. 22 ] - Combined immunodeficiency in a chilhood with Cd3 deficiency: case report
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