High molecular weight components from Ascaris suum inhibit the OVA specific response by a mechanism dependent on MYD88 adapter molecule (2006)
- Authors:
- Autor USP: MACEDO, MAHASTI SAHIHI DE - ICB
- Unidade: ICB
- Assunto: IMUNOLOGIA
- Language: Inglês
- Abstract: Introduction and Objectives: We have demonstrated that high molecular weight (P1) components from A. suum inhibit in vivo the expression of the molecules involved in antigen presentation in dendritic cells (DCs) from OVA+PI-primed mice and the ability of these cells to induce T cell proliferation compared to DC from OVA-primed mice. Here we evaluate the in vitro ability of PI to modulate the expression of MHC-II and co-stimulatory molecules by already activated CD11c+cells from OVA-immunized mice and the potential of these cells to stimulate OVA-specific T cells, plus the role of the Myd88 adapter protein in its in vivo suppressive effect. Methods and Results: Purified CD11c+ cells from mice immunized with OVA in CFA were incubated with OVA or OVA+P1 for 18 h, labeled with FITC- or PE-conjugated anti-MHC-II, -CD80, -CD86 or -CD40 mAbs, and analyzed by flow cytometry or cultured with OVA-specific T cells. We observed that PI suppressed the MHC-II and co-stimulatory molecules expression by CD11c+cells from OVA-immunized mice pulsed with OVA+P1 compared to that obtained on CD11c+ cells pulsed only with OVA. Reduction of 53% in the proliferation of OVA-specific T cells was obtained when they were cultured with CD11c+cells pulsed with OVA+PI. In addition, MHCII and co-stimulatory molecule expression was evaluated on lymph node cells from C57BL/6 WT or Myd88KO mice immunized with OVA or OVA+P1 in CFA. The expression of these molecules was reduced by 43-50%on cells from OVA+PI-primed WT mice compared to those from OVAprimed mice. In contrast, no difference was obtained in Myd88KO mice primed in either way. OVA-specific T cell proliferation was only suppressed when antigen-presenting cells form OVA+PI-immunized WT were used in culture. Conclusion: PI has a potent modulatory effect on the fenotype and function of DCs and this effect is related to Myd88 signaling pathway.
- Imprenta:
- Source:
- Título: Abstracts
- Conference titles: Meeting of the Brazilian Society for Immunology
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ABNT
SILVA, S R et al. High molecular weight components from Ascaris suum inhibit the OVA specific response by a mechanism dependent on MYD88 adapter molecule. 2006, Anais.. São Paulo: Instituto de Ciências Biomédicas, Universidade de São Paulo, 2006. . Acesso em: 10 jan. 2026. -
APA
Silva, S. R., Jacysyn, J. F., Macedo, M. S. de, & Faquim-Mauro, E. L. (2006). High molecular weight components from Ascaris suum inhibit the OVA specific response by a mechanism dependent on MYD88 adapter molecule. In Abstracts. São Paulo: Instituto de Ciências Biomédicas, Universidade de São Paulo. -
NLM
Silva SR, Jacysyn JF, Macedo MS de, Faquim-Mauro EL. High molecular weight components from Ascaris suum inhibit the OVA specific response by a mechanism dependent on MYD88 adapter molecule. Abstracts. 2006 ;[citado 2026 jan. 10 ] -
Vancouver
Silva SR, Jacysyn JF, Macedo MS de, Faquim-Mauro EL. High molecular weight components from Ascaris suum inhibit the OVA specific response by a mechanism dependent on MYD88 adapter molecule. Abstracts. 2006 ;[citado 2026 jan. 10 ] - Antigenic competition in ige antibody production. III. Supressive effect on th and b cells
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