'CD4 POT.+''CD25 POT.HIGH''FOXP3 POT.+' regulatory T cells in humans (2006)
- Authors:
- Autor USP: RIZZO, LUIZ VICENTE - ICB
- Unidade: ICB
- Assunto: IMUNOLOGIA
- Language: Inglês
- Abstract: Introduction and Aims: The human CD4+CD25+ T cell population contains a lineage of regulatory T (Treg) cells capable of modulating conventional immune responses. Most Treg cells are defined based on expression of CD4, high levels of CD25 and the transcription factor and critical mediator of their development and function, FOXP3. However, these markers have proven problematic for uniquely defining this specialized T cell subset in humans, in addition to the fact that it represents 1-4% of the total CD4+ circulating lymphocytes. The aim of our work is to establish a criteria to identify the Treg cell population in control individuals, to further determine if a diminished number or improper function of these cells could be responsible for the high incidence of autoimmunity and/or allergic symptoms observed in patients with Common Variable Immunodeficiency and Selective IgA deficiency. Methods: Human peripheral blood mononuclear cells were prepared by centrifugation over Isolymph gradients. For immunostaining, FITC-, PE- and APC- conjugated antibodies against human CD4, CD25, FOXP3 and the respective isotype controls were used. Quantitative analysis of CD4+CD25lowFOXP3+ and CD4+CD25highFOXP3+ Treg cells was performed by flow cytometry technique. Results are presented by median ± standard deviation. Results: We studied 7 males and 8 females with average age of 26,67 ± 3,81. Comparison of CD4+CD25HighFOXP3+ (73,90 ± 10,44) and CD4+CD25LowFOXP3+(49,70 ± 17,11) populationsby Mann-Whitney U test have demonstrated a significant statistical difference (p<0,0003) regarding FOXP3 expression. Conclusion: Human CD4+CD25+ T cells comprise a mixed population consisting of conventional activated T cells expressing CD25 as well as Treg cells. The human CD4+CD25high T cell population corresponds with the one that expresses high levels of FOXP3.
- Imprenta:
- Source:
- Título: Abstracts
- Conference titles: Meeting of the Brazilian Society for Immunology
-
ABNT
GENRE, J e ERRANTE, P R e RIZZO, Luiz Vicente. 'CD4 POT.+''CD25 POT.HIGH''FOXP3 POT.+' regulatory T cells in humans. 2006, Anais.. São Paulo: Instituto de Ciências Biomédicas, Universidade de São Paulo, 2006. . Acesso em: 12 mar. 2026. -
APA
Genre, J., Errante, P. R., & Rizzo, L. V. (2006). 'CD4 POT.+''CD25 POT.HIGH''FOXP3 POT.+' regulatory T cells in humans. In Abstracts. São Paulo: Instituto de Ciências Biomédicas, Universidade de São Paulo. -
NLM
Genre J, Errante PR, Rizzo LV. 'CD4 POT.+''CD25 POT.HIGH''FOXP3 POT.+' regulatory T cells in humans. Abstracts. 2006 ;[citado 2026 mar. 12 ] -
Vancouver
Genre J, Errante PR, Rizzo LV. 'CD4 POT.+''CD25 POT.HIGH''FOXP3 POT.+' regulatory T cells in humans. Abstracts. 2006 ;[citado 2026 mar. 12 ] - Combined immunodeficiency in a chilhood with Cd3 deficiency: case report
- Development of CD4+T cell lines that suppress an antigen-specific immune response in vivo
- Oral tolerance mechanisms reside on reduction of pathogenic cytokine secretion rather than on down-regulation of costimulatory molecules
- Influência de antígenos alogeneicos e singeneicos na geração de células T reguladoras
- Effects of cyclooxygenase inhibitors treatment in murine model of trypanosoma cruzi infection
- Administration of m. Leprae hsp65 aggravates experimental autoimmune uveitis [eau] in mice
- Characterization of T regulatory cells and cytokine profile in peripheral blood of patients with vogt-koyanagi-harada disease (VKH) and autoimmune uveitis
- Anti-inflammatory cytokines increase in peripheral blood from patients with Vogt-Koyanagi-Harada (VKH) is associated with the resolution phase of the disease
- Supressão da proliferação de células T MOG35-55 específicas pela expressão de ido em linhagem de células de microglia
- Pivotal role of 5-lipooxygenase in controlling tissue parasitism in the heart and oxidative stress during Trypanosoma cruzi infection
How to cite
A citação é gerada automaticamente e pode não estar totalmente de acordo com as normas
