Cyclophosphamide-mediated immunomodulatory effects:: an action on dendritic cells (DC)? (2006)
- Authors:
- Autor USP: BARBUTO, JOSE ALEXANDRE MARZAGAO - ICB
- Unidade: ICB
- Assunto: IMUNOLOGIA
- Language: Inglês
- Abstract: Introduction & Objectives: Cyclophosphamide (Cy) has been used as an immunomodulatory drug, capable of enhancing immune responses in vaccination protocols. However, its mechanisms of action remain unclear. One possibility would be that the Cy-induced hematopoietic response generates a more favourable environment for the differentiation of effective DC. To test this hypothesis we studied the in vitro differentiation of DC from blood monocytes obtained from cancer patients treated with Cy, in two vaccination protocols for advanced/metastatic cancer. Methods & Results: Patients that received 300 mg/m2 of Cy iv, three days before vaccination against their tumour donated peripheral blood mononuclear cells (PBMC) before Cy administration and three days thereafter. PBMC were separated by density gradient centrifugation and cultured for 7 days with IL-4 and GM-CSF. At day 5, TNF-a was added to the culture. At the end of the culture period, the expression of the CD1a, CD11c, HLADR, CD86, CD80, CD83, CCR7 and CD14 was evaluated by flow cytometry. The frequency of CD86+ cells in the cultures was significantly higher after Cy treatment (14.2% x 23.1%; p=0.002). Furthermore, non-vaccinated patients showed a significantly higher increase in CD86+ cell frequency than those previously vaccinated (p= 0.05). In one sample tested, cells differentiated from monocytes obtained after Cy treatment induced stronger allogeneic T lymphocyte proliferation than cellsderived from monocytes collected before treatment of the same patient (proliferation indexes of 2.53 x 1.39). Conclusions: These data support the hypothesis that Cy treatment affects PBMC ability to differentiate in response to DC differentiation stimuli. Such effect could contribute to the immune enhancing activity of this drug and should be considered in the design of future studies.
- Imprenta:
- Source:
- Título: Abstracts
- Conference titles: Meeting of the Brazilian Society for Immunology
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ABNT
CAVALCANTI, C M et al. Cyclophosphamide-mediated immunomodulatory effects:: an action on dendritic cells (DC)? 2006, Anais.. São Paulo: Instituto de Ciências Biomédicas, Universidade de São Paulo, 2006. . Acesso em: 24 jan. 2026. -
APA
Cavalcanti, C. M., Maluf, F., Buzaid, A. C., Martins, S. C., & Barbuto, J. A. M. (2006). Cyclophosphamide-mediated immunomodulatory effects:: an action on dendritic cells (DC)? In Abstracts. São Paulo: Instituto de Ciências Biomédicas, Universidade de São Paulo. -
NLM
Cavalcanti CM, Maluf F, Buzaid AC, Martins SC, Barbuto JAM. Cyclophosphamide-mediated immunomodulatory effects:: an action on dendritic cells (DC)? Abstracts. 2006 ;[citado 2026 jan. 24 ] -
Vancouver
Cavalcanti CM, Maluf F, Buzaid AC, Martins SC, Barbuto JAM. Cyclophosphamide-mediated immunomodulatory effects:: an action on dendritic cells (DC)? Abstracts. 2006 ;[citado 2026 jan. 24 ] - Imunoterapia do cancer
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