Macrophage subpopulations are differentially modulated by flagellin (2006)
- Autor USP: RUSSO, MOMTCHILO - ICB
- Unidade: ICB
- Assunto: IMUNOLOGIA
- Language: Inglês
- Abstract: Introduction and Objectives: Macrophages (MO) are a heterogeneous population of tissue cells that can trigger innate immune responses after stimulation with pathogen-associated molecular patterns (PAMPs). Many types of PAMPs receptors are expressed on MO and among them; the Toll-like receptors (TLRs) family has been extensively studied. The aim of this work was to evaluate the activation profile of peritoneal (PM) and alveolar (AM) MO subpopulations induced by Salmonella typhimurium flagellin (FliCi), a TLR5 agonist. Methods and Results: C57BL/6 mice were inoculated i.p. or i.n. with FliCi (1µ g) and 48h after, peritoneal (PEC) or bronchoalveolar (BAL) cells were collected. Injection of FliCi induced an increase in the MO numbers in both compartments ( Control (C) PEC: 10 ± 0.8; FliCi PEC: 19 ± 0.6; C BAL: 1.8 ± 0.2; FliCi BAL: 5.6 ± 0.5 x105 cells). We found that AM are constituted by a homogeneous population for F4/80 expression (F4/80int) and PM comprise two distinct subpopulations (F4/80hi and F4/80lo).Flow cytometry analysis indicated a positive modulation induced by FliCi on molecules involved with antigen presentation and cellular activation, in all MO subpopulations. Up regulation of CD80 expression was more pronounced in F4/80hi PM (C- 113.8; FliCi- 388.9), CD40 in F4/80lo PM (C- 22.1; FliCi- 65.9) and GR1 in F4/80int AM (C- 31.5; FliCi- 76.4), as mean fluorescence intensity revealed. F4/80hi PM was easilymodulated by FliCi to produce IL-10 (C-13.3%; FliCi- 33.4%), F4/80lo PM to produce IL-12 (C- 5.5%, FliCi- 7.6%) and F4/80int AM to TNF-á production (C- 5.96%; FliCi- 13.6%), as seen by the frequency of positive cells. iNOS expression up-regulation in response to FliCi was observed in all three subpopulations whereas only PM cells showed a robust NO production (C- 1.8 ± 0.6µ M; FliCi- 28.3 ± 3.3µ M). Conclusion: Our results indicate that FliCi is a strong stimulus that differentially affects distinct MO subpopulations, independently of their localization.
- Título do periódico: Abstracts
- Conference titles: Meeting of the Brazilian Society for Immunology
ABNTCASSADO, A; BUZZO, C; MASSIS, L; et al. Macrophage subpopulations are differentially modulated by flagellin. Anais.. São Paulo: [s.n.], 2006.
APACassado, A., Buzzo, C., Massis, L., Ferreira, L. C. S., Russo, M., & Bastos, K. R. (2006). Macrophage subpopulations are differentially modulated by flagellin. In Abstracts. São Paulo.
NLMCassado A, Buzzo C, Massis L, Ferreira LCS, Russo M, Bastos KR. Macrophage subpopulations are differentially modulated by flagellin. Abstracts. 2006 ;
VancouverCassado A, Buzzo C, Massis L, Ferreira LCS, Russo M, Bastos KR. Macrophage subpopulations are differentially modulated by flagellin. Abstracts. 2006 ;
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