Prostaglandins (PG) role in the effects of ethanol (ETOH) upon dendritic cells (DCS) (2006)
- Autor USP: BARBUTO, JOSE ALEXANDRE MARZAGAO - ICB
- Unidade: ICB
- Assunto: IMUNOLOGIA
- Language: Inglês
- Abstract: Introduction and objective: DCs are the main antigen presenting cells (APCs). They capture, process and present antigens to T lymphocytes and play a central role in the induction and maintenance of immune responses. When DCs are differentiated and matured in presence of EtOH they acquire an inhibitory phenotype that may cause T cell anergy. Here we investigated if these modifications of DCs phenotype by EtOH depended on PG synthesis within the cultures. Methods and Results: Adherent cells separated from peripheral blood mononuclear cells (PBMC) were cultured in RPMI-1640 medium, supplemented with 10% fetal calf serum, GM-CSF and IL-4 (50ng/mL each) for 7 days. On the 5th day, TNF-a (50ng/mL) was added for DC activation. On the 1st day, EtOH (1 g/L) was added to the cultures and on 3rd and 5th days, cultures were suplemented with half the EtOH initial concentration. Indomethacin, 10 mM, (INDO) was added to the cultures either on the 1st, 3rd or 5th day.On the 7th day, cells were harvested and analyzed by flow cytometry for the expression of CD80, CD86, CD1a, CD11c, HLA-DR, CD83, CD14, CD123 and CD11c. All markers analyzed were affected by both EtOH and INDO treatments, but the most striking differences were found in the percentage of CD86+ CD80+ cells: 61% in the control group, 44 % in the EtOH group, 20% in the INDO 1st day group, 56% in the INDO 5th day group., The addition of INDO on the 5th day to EtOH treatedcells, suppressed the effect of EtOH upon the cells, increasing the percentage of double positive cells to 54%. Interestingly, the addition of INDO on the first day did not have the same effect (40% CD80+CD86+ cells). Conclusion: These data suggest that PGs may be, indeed, involved in EtOH effects upon DC differentiation and maturation in vitro. The difference between the addition of INDO between the first and 5th day may be related to a possible necessary role of PGs in the "physiological" differentiation of DCs.
- Título do periódico: Abstracts
- Conference titles: Meeting of the Brazilian Society for Immunology
ABNTOLIVEIRA, B L; BARBUTO, José Alexandre Marzagão. Prostaglandins (PG) role in the effects of ethanol (ETOH) upon dendritic cells (DCS). Anais.. São Paulo: [s.n.], 2006.
APAOliveira, B. L., & Barbuto, J. A. M. (2006). Prostaglandins (PG) role in the effects of ethanol (ETOH) upon dendritic cells (DCS). In Abstracts. São Paulo.
NLMOliveira BL, Barbuto JAM. Prostaglandins (PG) role in the effects of ethanol (ETOH) upon dendritic cells (DCS). Abstracts. 2006 ;
VancouverOliveira BL, Barbuto JAM. Prostaglandins (PG) role in the effects of ethanol (ETOH) upon dendritic cells (DCS). Abstracts. 2006 ;
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