Allergic lung grafts induce acumulation of hemopoietic cells in lungs and increase the response of bone-marrow cells to IL-5 in a ectopic transplantation model (2006)
- Authors:
- Autor USP: VARGAFTIG, BERNARDO BORIS JORGE - ICB
- Unidade: ICB
- Assunto: FARMACOLOGIA
- Language: Inglês
- Abstract: Introduction and Objectives: Airway challenge of ovalbumine-sensitized mice induces intrapulmonary accumulation of eosinophil progenitors. To evaluate the relative contributions of systemic mediator release and of immunological lung injury, to the accumulation of hemopoietic cells in murine lungs and their effects in hemopoietic cells of bone marrow, we developed a transplantation model. Methods and Results: Lung fragments from OVA-sensitized and -challenged BALB/c and CBA mice, or from CBA/Ca mice transgenic for murine IL-5, were implanted ectopically in recipients which were either naive or OVA-sensitized (but not challenged), and hemopoietic cells in recipients' lungs and bone-marrow were analyzed 24 h after transplantation ("n" for all experiments is 5 or more). In BALB/c mice, accumulation of progenitors in lung (29.08 ± 4.2 colonies/lung) and increased response to IL-5 in bone-marrow hemopoietic cells (47.5 ± 5.2% EPO+ cells) occurred only when: a) donors were sensitized and airway-challenged with homologous allergen; b) recipients were sensitized.Grafts from the appropriate donors released biologically active IL-5, which was effective in sensitized recipients (600pg/mL). The effect of the appropriate donor-recipient combination was prevented by neutralizing anti-IL-5 antibody (90.33 ± 3.57% of inhibition). Grafts from unchallenged, sensitized donors synergized with recombinant IL-5 in sensitized recipients (146.67 ± 9.4colonies/lung). Unlike BALB/c, grafts from naive IL-5 transgenic CBA/Ca mice (whose lungs contained a large number of progenitors, independently of sensitization and challenge) were effective in nontransgenic, ovalbumin-sensitized recipients (88.62 ± 29.1 colonies/lung). Conclusion: These results show that: a) intrapulmonary accumulation of progenitors is independent of immunological injury; b) grafts systemically release IL-5, which is required for progenitor accumulation in the recipients' own lungs; c) sensitization is required for full responsiveness to IL-5 and for generation of lung-derived signals which synergize with IL-5.
- Imprenta:
- Source:
- Título: Abstracts
- Conference titles: Meeting of the Brazilian Society for Immunology
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ABNT
QUETO, T et al. Allergic lung grafts induce acumulation of hemopoietic cells in lungs and increase the response of bone-marrow cells to IL-5 in a ectopic transplantation model. 2006, Anais.. São Paulo: Instituto de Ciências Biomédicas, Universidade de São Paulo, 2006. . Acesso em: 26 jan. 2026. -
APA
Queto, T., Elsas, M. I. G., Joseph, D., Maximiano, E. S., Barradas, M. G., Gardel, M. A., et al. (2006). Allergic lung grafts induce acumulation of hemopoietic cells in lungs and increase the response of bone-marrow cells to IL-5 in a ectopic transplantation model. In Abstracts. São Paulo: Instituto de Ciências Biomédicas, Universidade de São Paulo. -
NLM
Queto T, Elsas MIG, Joseph D, Maximiano ES, Barradas MG, Gardel MA, Elsas PX, Vargaftig BB. Allergic lung grafts induce acumulation of hemopoietic cells in lungs and increase the response of bone-marrow cells to IL-5 in a ectopic transplantation model. Abstracts. 2006 ;[citado 2026 jan. 26 ] -
Vancouver
Queto T, Elsas MIG, Joseph D, Maximiano ES, Barradas MG, Gardel MA, Elsas PX, Vargaftig BB. Allergic lung grafts induce acumulation of hemopoietic cells in lungs and increase the response of bone-marrow cells to IL-5 in a ectopic transplantation model. Abstracts. 2006 ;[citado 2026 jan. 26 ] - Dendritic cells recruited to the lungs shortly after intranasal delivery of Mycobacterium bovis BCG drive the primary immune response towards a TH1-type cytokine production
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