Involvement of cytochrome C and SMAC/DIABLO release from mitochondria and caspase -3 and -6 activation in fatty acid- induced apoptosis in human blood lymphocytes (2006)
- Authors:
- Autor USP: CURI, RUI - ICB
- Unidade: ICB
- Assunto: FISIOLOGIA
- Language: Português
- Abstract: Treatment with a mixture of fatty acids in similar proportion to that found in human plasma triggers apoptosis in peripheral blood lymphocytes from healthy subjects. The present study was undertaken to evaluate if the effect of a mixture of fatty acids upon normal human lymphocytes involves cytochrome c and Smac/Diablo release from the mitochondria, activation of caspases-3, -6 and -8 and expression of the anti-apoptotic blc-2 and 14 3 3 and the pro-apoptotic bax and FasL proteins. Peripheral blood lymphocytes from healthy subjects were isolated and treated for 24 h with increasing concentrations (0.2 and 0.4 mM) of the fatty acid mixture. Afterwards, cells were harvested and the cytochrome c and Smac/Diablo release from mitochondrial intermembrane space into cytosol and expression of blc-2, 14 3 3, FasL and bax were investigated by Western blotting analysis. Activities of caspases-3, -6 and -8 were determined by using spectrofluorometric assays. Lymphocytes treated with the fatty acid mixture presented increased cytochrome c and Smac/Diablo release from mitochondria as compared with control lymphocytes without treatment. Caspase -3 and -6 activities were increased by 146% and 22%, respectively, by the treatment with 0.4 mM concentration of the fatty acid mixture for 24 h. The expression of bcl-2 protein was significantly reduced in lymphocytes treated with the fatty acid mixture, by 40% at 0.2 mM and by 80% at 0.4 mM fatty acid concentration as well as 14 3 3 proteinthat was decreased by 66% at 0.2 mM and by 23% at 0.4 mM. However, bax and FasL proteins were not affected by the fatty acid treatment. In conclusion, fatty acids induce apoptosis in human lymphocytes through cytochrome c and Smac/Diablo release from the mitochondria, activation of the caspase cascade and by decrease of anti-apoptotic bcl-2 and 14 3 3 proteins content.
- Imprenta:
- Publisher: Federação de Sociedades de Biologia Experimental
- Publisher place: Águas de Lindóia
- Date published: 2006
- Source:
- Título do periódico: Programa e resumos
- Conference titles: Reunião Anual da Federação de Sociedades de Biologia Experimental - FESBE
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ABNT
CAMPOIO, T. R. et al. Involvement of cytochrome C and SMAC/DIABLO release from mitochondria and caspase -3 and -6 activation in fatty acid- induced apoptosis in human blood lymphocytes. 2006, Anais.. Águas de Lindóia: Federação de Sociedades de Biologia Experimental, 2006. . Acesso em: 24 abr. 2024. -
APA
Campoio, T. R., Batista, T. S., Braga, S. B., Barbosa, S., Pires, J., Curi, R., & Otton, R. (2006). Involvement of cytochrome C and SMAC/DIABLO release from mitochondria and caspase -3 and -6 activation in fatty acid- induced apoptosis in human blood lymphocytes. In Programa e resumos. Águas de Lindóia: Federação de Sociedades de Biologia Experimental. -
NLM
Campoio TR, Batista TS, Braga SB, Barbosa S, Pires J, Curi R, Otton R. Involvement of cytochrome C and SMAC/DIABLO release from mitochondria and caspase -3 and -6 activation in fatty acid- induced apoptosis in human blood lymphocytes. Programa e resumos. 2006 ;[citado 2024 abr. 24 ] -
Vancouver
Campoio TR, Batista TS, Braga SB, Barbosa S, Pires J, Curi R, Otton R. Involvement of cytochrome C and SMAC/DIABLO release from mitochondria and caspase -3 and -6 activation in fatty acid- induced apoptosis in human blood lymphocytes. Programa e resumos. 2006 ;[citado 2024 abr. 24 ] - Toxicidade de ácidos graxos em células produtoras de insulina HIT-T15
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