Expression of metalloproteinase 2 in the cell response to poros demineralized bovine bone matrix (2005)
- Authors:
- USP affiliated authors: LAURIS, JOSÉ ROBERTO PEREIRA - FOB ; TAGA, RUMIO - FOB
- Unidade: FOB
- Subjects: METALOPROTEINASES; MACRÓFAGOS; IMPLANTE ÓSSEO ANIMAL
- Language: Inglês
- Abstract: The purpose of the study was to analyze the involvement of metalloproteinase 2 (MMP-2) and macrophages in the tissue and cell response to the organic graft material produced from bovine cancellous bone. Thirty adult male white Wistar rats (Rattus norvegicus) received implants of blocks of demineralized bovine bone matrix between the fasciae of the quadriceps muscle. The specimens collected at 3, 7, 14, 21 and 28 days after implantation (n = 6/period). Sections of 6 µm thick were stained with hematoxylin and eosin and immunolabeled with anti-MMP-2 and anti-CD68 using standard avidin-biotin-peroxidase method. The tissue response to the material was initially mediated by polymorphonuclear neutrophils, evolving to a mononuclear inflammatory infiltrate with macrophages and few lymphocytes and plasma cells and presence of inflammatory multinucleated giant cells (GC) in contact with the material that exhibited signs of resorption. The number of cells immunolabeled to MMP-2 was highest at day 7 (103.2 ± 39.1), but significantly decreased (F = 3.67; p = 0.044) until day 28 (45.9 ± 13.1). CD68 immunostaining also significantly decreased (F = 6.75; p = 0.007) from day 7 (49.5 ± 10.4) to day 28 (19.5 ± 8.9). A positive and statistically significant correlation was observed between the evolutions of these two variables. The material had been almost completely resorbed at day 28. Among cells present at the granuloma, anti-MMP-2 immunostaining was predominant and more intense inmacrophages, yet lightly immunolabeled multinucleated giant cells were found in close contact with the material. Thus, considering the experimental limitations of this study, we concluded that MMP-2 produced by macrophages participates in the resorption of demineralized bovine bone.
- Imprenta:
- Source:
- Título: Journal of Molecular Histology
- ISSN: 1567-2379
- Volume/Número/Paginação/Ano: v. 36, n. 4, p. 311-316, May 2005
-
ABNT
MENDONÇA, Thaís Accorsi et al. Expression of metalloproteinase 2 in the cell response to poros demineralized bovine bone matrix. Journal of Molecular Histology, v. 36, n. 4, p. 311-316, 2005Tradução . . Disponível em: http://www.springerlink.com/media/ecxx5u6vvpdwtnfjhq06/contributions/b/2/4/2/b2422417k4755712.pdf. Acesso em: 31 dez. 2025. -
APA
Mendonça, T. A., Zambuzzi, W. F., Paiva, K. B. da S., Lauris, J. R. P., Cestari, T. M., Taga, R., & Granjeiro, J. M. (2005). Expression of metalloproteinase 2 in the cell response to poros demineralized bovine bone matrix. Journal of Molecular Histology, 36( 4), 311-316. Recuperado de http://www.springerlink.com/media/ecxx5u6vvpdwtnfjhq06/contributions/b/2/4/2/b2422417k4755712.pdf -
NLM
Mendonça TA, Zambuzzi WF, Paiva KB da S, Lauris JRP, Cestari TM, Taga R, Granjeiro JM. Expression of metalloproteinase 2 in the cell response to poros demineralized bovine bone matrix [Internet]. Journal of Molecular Histology. 2005 ; 36( 4): 311-316.[citado 2025 dez. 31 ] Available from: http://www.springerlink.com/media/ecxx5u6vvpdwtnfjhq06/contributions/b/2/4/2/b2422417k4755712.pdf -
Vancouver
Mendonça TA, Zambuzzi WF, Paiva KB da S, Lauris JRP, Cestari TM, Taga R, Granjeiro JM. Expression of metalloproteinase 2 in the cell response to poros demineralized bovine bone matrix [Internet]. Journal of Molecular Histology. 2005 ; 36( 4): 311-316.[citado 2025 dez. 31 ] Available from: http://www.springerlink.com/media/ecxx5u6vvpdwtnfjhq06/contributions/b/2/4/2/b2422417k4755712.pdf - Quantitative age-related differences in human sublingual gland
- Orthodontic movement in bone defects fillet with xenograft
- Relation between sublingual glands and mouth floor folds in edentulous
- Estudo morfologico, morfometrico, topoquimico subcelular e em replicas de criofratura da celula acinosa pancreatica do rato durante a vida fetal
- Análise alométrica do desenvolvimento pós-natal da glândula submandibular de rato
- Resorption of biocompatible membranes of bovine cortical bone into cortical bone with exposed medullar tissue
- Estudo estereológico da evolução do volume nuclear e citoplasmático de células acinosas e de ductos granulosos de glândulas submandibulares de ratos durante a vida pós-natal
- Defeito ósseo de tamanho crítico: um modelo experimental para o teste de biomateriais
- Estudo morfometrico da evolucao do volume nuclear e citoplasmatico de celulas mucosas e serosas de glandulas sublinguais do rato durante a vida pos-natal inicial
- Modificações nas populações celulares durante a osteogênese ectópica induzida por matriz óssea alogênica
How to cite
A citação é gerada automaticamente e pode não estar totalmente de acordo com as normas
