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Production and secretion of heat labile toxins by enterotoxigenic Escherichia coli strains isolated from human and porcine hosts (2005)

• Authors:
  • Rodrigues, Juliana Falcão
  • Lásaro, M. A. S.
  • Castro, Antonio Fernando Pestana de
  • Ferreira, L. C. S.
• Autor USP: CASTRO, ANTONIO FERNANDO PESTANA DE - ICB
• Unidade: ICB
• Assunto: MICROBIOLOGIA
• Language: Inglês
• Abstract: Enterotoxigenic Escherichia coli (ETEC) represent one of the most important etiological agents of diarrhea in developing countries. Ability to induce disease is characteristically associated with production of colonization factors and at least one of two enterotoxins: the heat-labile toxin (LT) and the heat-stable toxin (ST). Previous studies have indicated that LT produced by ETEC strains isolated from humans (LTh) is distinct of the toxin produced by ETEC isolated from pigs (LTp) with regard to immunological properties, isoeletric point, electrophoretic mobility on SDS-PAGE gel, and amino acid composition. However, the capacity to produce and secrete LTh or LTp by ETEC strains was not previously investigated. In this work we determined the production and secretion of LT in a set of 42 ETEC strains isolated from piglets or humans. The LT concentrations detected in whole cell extracts or in culture supernatants were determined with a sandwich ELISA based on LT-specific antibodies. The LT detected in crude extract of ETEC strains isolated from human hosts ranged from 29.1 to 2,776.6 ng/ml while the toxin secretion ranged from 0 % to 53% of the total toxin produced. On the other hand, the LT levels expressed by ETEC strains isolated from porcine hosts ranged from 9 to 300 ng/ml, while LT secretion varied from 0% to 20% of the toxin produced. These results indicate that the capacity to produce and secrete LT by ETEC strains isolated from human and porcine hosts may varygreatly according to the strain analyzed. Moreover, the present evidences suggest that the natural diversity of wild ETEC to express and secrete LT involves both gene regulation and toxin secretion mechanisms
• Imprenta:
  • Publisher: Comissão de Cultura e Extensão Universitária do ICB/USP
  • Publisher place: São Paulo
  • Date published: 2005
• Source:
  • Título do periódico: Resumos
• Conference titles: Congresso do Instituto de Ciências Biomédicas

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How to cite

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• ABNT

  RODRIGUES, Juliana Falcão; LÁSARO, M. A. S.; CASTRO, Antonio Fernando Pestana de; FERREIRA, L. C. S. Production and secretion of heat labile toxins by enterotoxigenic Escherichia coli strains isolated from human and porcine hosts. Anais.. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2005.

• APA

  Rodrigues, J. F., Lásaro, M. A. S., Castro, A. F. P. de, & Ferreira, L. C. S. (2005). Production and secretion of heat labile toxins by enterotoxigenic Escherichia coli strains isolated from human and porcine hosts. In Resumos. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP.

• NLM

  Rodrigues JF, Lásaro MAS, Castro AFP de, Ferreira LCS. Production and secretion of heat labile toxins by enterotoxigenic Escherichia coli strains isolated from human and porcine hosts. Resumos. 2005 ;

• Vancouver

  Rodrigues JF, Lásaro MAS, Castro AFP de, Ferreira LCS. Production and secretion of heat labile toxins by enterotoxigenic Escherichia coli strains isolated from human and porcine hosts. Resumos. 2005 ;

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