Angiotensin type 2 receptor (AT2) expression in thyroid hormone-induced hypertrophy in primary cultured of cardiac myocytes (2005)
- Authors:
- Autor USP: CHAVES, MARIA LUIZA MORAIS BARRETO DE - ICB
- Unidade: ICB
- Assunto: ANATOMIA
- Language: Inglês
- Abstract: Objectives: Renin-Angiotensin System (RAS) has been associated with cardiac hypertrophy induced by thyroid hormone. However, few is known regarding the regulation of Angiotensin receptors in cardiac cells after hyperthyroidism condition. Methods: Newborn rat ventricular cardiomyocytes were obtained from 1 to 2 day old Wistar rats. After successive digestion of tissue, cardiomyocytes were separated using a Percoll gradient and pooled in DMEM enriched with Newborn Calf and Horse Serum. 24 hours before the treatment, cells were maintained in serum free media. Cells were treated with crescent doses of T3 (1ηM - 1μM) during 24 hours. After that,total RNA was obtained from cardiomyocytes and the ANF and AT2 gene expression were evaluated using semiquantitative RT-PCR. The ANF has been used for literature as a reliable cardiac hypertrophy marker. Results: After treatment, cardiomyocytes presented a significant increase on ANF expression (up to 76%) showing the hypertrophic state of these cells. However, the AT2 expression was significantly decreased after treatment with T3, characterizing a dose-response relation, which showed the lowest expression level with 0.1μM of T3 (21.43%). Conclusion: The effect of Thyroid hormone on decreasing AT2 receptors levels on cardiac myocytes suggests an important role of hormone on local/cardiac RAS regulation
- Imprenta:
- Publisher: Comissão de Cultura e Extensão Universitária do ICB/USP
- Publisher place: São Paulo
- Date published: 2005
- Source:
- Título: Resumos
- Conference titles: Congresso do Instituto de Ciências Biomédicas
-
ABNT
CARNEIRO-RAMOS, M. S. e DINIZ, Gabriela Placoná e BARRETO-CHAVES, Maria Luiza Morais. Angiotensin type 2 receptor (AT2) expression in thyroid hormone-induced hypertrophy in primary cultured of cardiac myocytes. 2005, Anais.. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2005. . Acesso em: 17 mar. 2026. -
APA
Carneiro-Ramos, M. S., Diniz, G. P., & Barreto-Chaves, M. L. M. (2005). Angiotensin type 2 receptor (AT2) expression in thyroid hormone-induced hypertrophy in primary cultured of cardiac myocytes. In Resumos. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP. -
NLM
Carneiro-Ramos MS, Diniz GP, Barreto-Chaves MLM. Angiotensin type 2 receptor (AT2) expression in thyroid hormone-induced hypertrophy in primary cultured of cardiac myocytes. Resumos. 2005 ;[citado 2026 mar. 17 ] -
Vancouver
Carneiro-Ramos MS, Diniz GP, Barreto-Chaves MLM. Angiotensin type 2 receptor (AT2) expression in thyroid hormone-induced hypertrophy in primary cultured of cardiac myocytes. Resumos. 2005 ;[citado 2026 mar. 17 ] - Maternal hyperthyroidism increases the susceptibility of rat adult offspring to cardiovascular disorders
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