Biphasic effect of inoculation of schwann cells on the combined behavior score (CBS) of nyu impactor contuse lesioned spinal cord rats is altered when cells are pre-treated with TNF-alfa. (2005)
- Authors:
- Autor USP: CHADI, GERSON - ICB
- Unidade: ICB
- Assunto: ANATOMIA
- Language: Português
- Abstract: Objetivo: It is known that the cytokines modulate the repair-promoted effects of Schwann cells (SC) on injured nerves probably by triggering the expression of neurotrophic molecules. Cultured SC also induce regeneration of lesioned spinal cord which can also be modulated byinflammatory cytokines. On the other hand, pro-inflamtory cytokines may impar the process of regeneration by interfing with myelin formation and fiber growth. The aim of the present study was to investigate whether the cytokine tumor necrosis factor alpha (TNF-a) can modify the ability of primary cultured SC to induce motor recover on NYU Impactor contuse lesioned rat spinal cord. Métodos e Resultados: Material and Methods: Primary cultured SC were pre-treated with TNF-a(30ng/ml) for 30 min or not. Young rats were submitted to a laminectomy (T10-11) and spinal cord received a moderate contuse lesion by means of a 25 cm drop of a 10 g rod promoted by the Impactor. Sham animals received laminectomy without cord lesion. Lesioned rats received injections of SC, with or without pre treatment with TNF-a. A volume of 10 ml SC (100,000 to 150,000/ml of cultured medium) was injected stereotaxically in the epicenter of the cord lesion immediately after injury.) Sham rats received local injection of culture medium alone. The functional recovery of the hindlimbs of the rats was accessed through a combined behavior score (CBS) during 8 weeks. Results: Impactor parameters demonstrated the moderate contuselesion applied in the spinal cord. The CBS score show a better hindlimb function in the rats treated with SC alone 48hs, 72hs and 1 week after surgery as well at the 5th, 6th, 7th, and 8th weeks post injury compared with the lesioned rats that were not treated with SC. The above described effect was observed in the lesioned rats that received SC pre-treated with TNF-a only at 48 hours time-interval. Conclusões: It is concluded that SC inoculation in contuse lesioned spinal cord improves hindlimb motor function. The manipulation of the SC to increase the ability of the cells to promote functional recovery needs to be further investigated.
- Imprenta:
- Publisher: Federação de Sociedades de Biologia Experimental
- Publisher place: Águas de Lindóia, São Paulo
- Date published: 2005
- Source:
- Título do periódico: Resumos
- Conference titles: Reunião Anual da Federação de Sociedades de Biologia Experimental, FeSBE
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ABNT
GUZEN, F. P. et al. Biphasic effect of inoculation of schwann cells on the combined behavior score (CBS) of nyu impactor contuse lesioned spinal cord rats is altered when cells are pre-treated with TNF-alfa. 2005, Anais.. Águas de Lindóia, São Paulo: Federação de Sociedades de Biologia Experimental, 2005. . Acesso em: 23 abr. 2024. -
APA
Guzen, F. P., de Luca, B. A., Cunha, J. C., Taneda, M., Andrade, M. S. R., Gomide, V. C., & Chadi, G. (2005). Biphasic effect of inoculation of schwann cells on the combined behavior score (CBS) of nyu impactor contuse lesioned spinal cord rats is altered when cells are pre-treated with TNF-alfa. In Resumos. Águas de Lindóia, São Paulo: Federação de Sociedades de Biologia Experimental. -
NLM
Guzen FP, de Luca BA, Cunha JC, Taneda M, Andrade MSR, Gomide VC, Chadi G. Biphasic effect of inoculation of schwann cells on the combined behavior score (CBS) of nyu impactor contuse lesioned spinal cord rats is altered when cells are pre-treated with TNF-alfa. Resumos. 2005 ;[citado 2024 abr. 23 ] -
Vancouver
Guzen FP, de Luca BA, Cunha JC, Taneda M, Andrade MSR, Gomide VC, Chadi G. Biphasic effect of inoculation of schwann cells on the combined behavior score (CBS) of nyu impactor contuse lesioned spinal cord rats is altered when cells are pre-treated with TNF-alfa. Resumos. 2005 ;[citado 2024 abr. 23 ] - On the presence of the bfgf immunoreactivity in the spinal cord and dorsal root ganglia of the adult rat
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