CaSPREDICTOR: a new computer-based tool for caspase substrate prediction (2005)
- Authors:
- Autor USP: BELIZARIO, JOSE ERNESTO - ICB
- Unidade: ICB
- Assunto: FARMACOLOGIA
- Language: Português
- Abstract: Objetivo: Screening and validation of putative caspase substrates using CaSpredictor software. Métodos e Resultados: We have chosen an arbitrary CASP score = 0.57, where the sensitivity is 60% and specificity is 97% as a threshold score for prediction. The program detected potential cleavage sites in the primary sequences of 1644 proteins in a dataset containing 9986 protein entries. Most of these putative caspase substrates are cytoplasmatic proteins (38%) and function in the signaling transduction patways (69%). Another set of plasma membrane proteins (26%) can be included in this group because they act as transmembrane receptors. However, typical apoptosis-related proteins cleaved by caspase, including DNA repair and structural proteins are poorly represented (Fig. 6). In the list presented are the proteins: ATP-binding cassette-1 (ABC-1), protein kinase akt-3, atonal protein homolog 1, PDZ-RhoGEF, atrophin- 1 interactin protein 1 and ADAM 17.We analyzed the interaction of putative substrates (score >0.57) via C14 caspase domain and found that the 11.5% (95/836) shared potential domain of interactions. Using the BLAST option at the Apoptosis Database we also detected a apoptosis related domain in 40% (38/95) of putative substrates. Conclusões: The caspase substrate database generated by CaSPredictor is available at site (http://icb.usp.br/`~farmaco/Jose). The relevant substrates might provide new insights link caspase cleavage to important apoptotic andnon-apoptotic biological processes
- Imprenta:
- Publisher: Federação de Sociedades de Biologia Experimental
- Publisher place: Águas de Lindóia, São Paulo
- Date published: 2005
- Source:
- Título: Resumos
- Conference titles: Reunião Anual da Federação de Sociedades de Biologia Experimental
-
ABNT
BELIZARIO, Jose Ernesto et al. CaSPREDICTOR: a new computer-based tool for caspase substrate prediction. 2005, Anais.. Águas de Lindóia, São Paulo: Federação de Sociedades de Biologia Experimental, 2005. . Acesso em: 27 jan. 2026. -
APA
Belizario, J. E., Garay-Malpartida, H. M., Alves, J., & Occiucci, J. (2005). CaSPREDICTOR: a new computer-based tool for caspase substrate prediction. In Resumos. Águas de Lindóia, São Paulo: Federação de Sociedades de Biologia Experimental. -
NLM
Belizario JE, Garay-Malpartida HM, Alves J, Occiucci J. CaSPREDICTOR: a new computer-based tool for caspase substrate prediction. Resumos. 2005 ;[citado 2026 jan. 27 ] -
Vancouver
Belizario JE, Garay-Malpartida HM, Alves J, Occiucci J. CaSPREDICTOR: a new computer-based tool for caspase substrate prediction. Resumos. 2005 ;[citado 2026 jan. 27 ] - Dermcidin differentially regulates the expression of EGF/ErbB family members in breast cancer cells
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