Comparative toxicity of oleic and linoleic acid on human lymphocytes (2005)
- Authors:
- Autor USP: CURI, RUI - ICB
- Unidade: ICB
- Assunto: FISIOLOGIA
- Language: Português
- Abstract: Objetivo: Commercially available lipid emulsions for parenteral nutrition are mainly composed by long chain triacylglycerol containing a high proportion of _-6 polyunsaturated fatty acids or _-9 monounsaturated fatty acids. The immunological impact of such therapy is particularly important because parenteral diets are often administered to critically ill patients as a mechanism to supply adequate nutrition during catabolic stress response. The comparative toxicity of oleic acid (OA) and linoleic acid (LA) on human lymphocytes and the type of cell death induced by these fatty acids were determined in vitro. Métodos e Resultados: Parameters of cell death were investigated by flow cytometric: cell viability, DNA fragmentation, phophatidylserine externalization, mitochondrial depolarization, neutral lipid accumulation and production of reactive oxygen species; by fluorescence microscopy: chromatin condensation. Additionally we employed a spectrofluorometric assay to determine the activities of caspase - 3, 6 and 8. Evidence is presented herein that OA is less toxic to human lymphocytes than LA. However, both fatty acids promoted apoptosis and necrosis of these cells. The mechanism of cell death induced by OA involved activation of caspase 3 while the mechanism of death induced by LA involved mitochondrial depolarization and ROS production. Importantly, neutral lipid accumulation may be a mechanism to protect lymphocytes against the toxicity induced by OA. Conclusões: OA may offer an immunological less problematic alternative to LA with respect to fatty acid supplementation of parenteral nutritional emulsions
- Imprenta:
- Publisher: Federação de Sociedades de Biologia Experimental
- Publisher place: Águas de Lindóia, São Paulo
- Date published: 2005
- Source:
- Título: Resumos
- Conference titles: Reunião Anual da Federação de Sociedades de Biologia Experimental, FeSBE
-
ABNT
CURY-BOAVENTURA, Maria Fernanda et al. Comparative toxicity of oleic and linoleic acid on human lymphocytes. 2005, Anais.. Águas de Lindóia, São Paulo: Federação de Sociedades de Biologia Experimental, 2005. . Acesso em: 07 out. 2024. -
APA
Cury-Boaventura, M. F., Gorjão, R., Lima, T. M., & Curi, R. (2005). Comparative toxicity of oleic and linoleic acid on human lymphocytes. In Resumos. Águas de Lindóia, São Paulo: Federação de Sociedades de Biologia Experimental. -
NLM
Cury-Boaventura MF, Gorjão R, Lima TM, Curi R. Comparative toxicity of oleic and linoleic acid on human lymphocytes. Resumos. 2005 ;[citado 2024 out. 07 ] -
Vancouver
Cury-Boaventura MF, Gorjão R, Lima TM, Curi R. Comparative toxicity of oleic and linoleic acid on human lymphocytes. Resumos. 2005 ;[citado 2024 out. 07 ] - Identificação de genes regulados pelo ácido oléico em células jurkat
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